BTC tetrapotassium
BTC tetrapotassium is a cell-impermeant calcium indicator (Kd approximately 7-26 µM) featuring many desirable properties for cellular calcium imaging, including long excitation wavelengths (400/485 nm), low sensitivity to Mg2+, and accuracy of ratiometric measurement.
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- CAS No.: 216453-54-2
- Formule: C33H25K4N3O12S
- Masse moléculaire:844.02
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Activité biologique
Description
In Vitro
BTC tetrapotassium can be introduced into cells using methods such as patch clamping and microinjection.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 216453-54-2
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Masse moléculaire 844.02
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Formule C33H25K4N3O12S
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SMILES
O=C(O[K])CN(C(C=C(OC1=O)C(C=C1C2=NC3=CC=CC=C3S2)=C4)=C4OCCOC(C=C5C)=C(C=C5)N(CC(O[K])=O)CC(O[K])=O)CC(O[K])=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Ca2+ Staining Technique
Ca2+ staining is an experimental technique that utilizes specific fluorescent probes (such as Fluo-4 AM, Fura-2, etc.) to qualitatively or quantitatively detect dynamic changes in intracellular Ca2+ concentrations; this is achieved by monitoring the changes in fluorescent signals generated when these probes bind to free intracellular calcium ions. The underlying principle relies primarily on the presence of chelating groups within the probe's molecular structure that possess high affinity for calcium ions.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)