DDO-2213
Based on 1 Customer Validation
DDO-2213 is an orally active and potent WDR5-MLL1 inhibitor, with an IC50 of 29 nM and a Kd value of 72.9 nM for the WDR5 protein. DDO-2213 selectively inhibits MLL (mixed lineage leukemia) histone methyltransferase activity and the proliferation of MLL translocation-harboring cells. DDO-2213 can be used for MLL fusion leukemia research.
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- Pureté : 98.84%
- CAS No.: 2378768-36-4
- Formule: C24H27ClFN7O
- Masse moléculaire:483.97
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HUVEC | IC50 |
>100 μM
Compound: 63; DDO-2213
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Antiproliferative activity against human HUVEC cells assessed as cell viability incubated for 72 hrs by CCK8 assay
Antiproliferative activity against human HUVEC cells assessed as cell viability incubated for 72 hrs by CCK8 assay
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[PMID: 34105966] |
| K562 | IC50 |
>100 μM
Compound: 63; DDO-2213
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Antiproliferative activity against human K562 cells assessed as cell viability incubated for 72 hrs by CCK8 assay
Antiproliferative activity against human K562 cells assessed as cell viability incubated for 72 hrs by CCK8 assay
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[PMID: 34105966] |
| MOLM-13 | GI50 |
13.09 μM
Compound: DDO-2213
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Antiproliferative activity against human MOLM-13 cells assessed as inhibition of cell proliferation measured after 5 days by ATPlite assay
Antiproliferative activity against human MOLM-13 cells assessed as inhibition of cell proliferation measured after 5 days by ATPlite assay
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[PMID: 34283608] |
| MOLM-13 | IC50 |
13.1 μM
Compound: 63; DDO-2213
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Antiproliferative activity against human MOLM-13 cells assessed as cell viability incubated for 72 hrs by CCK8 assay
Antiproliferative activity against human MOLM-13 cells assessed as cell viability incubated for 72 hrs by CCK8 assay
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[PMID: 34105966] |
| MV4-11 | GI50 |
12.57 μM
Compound: DDO-2213
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Antiproliferative activity against human MV4-11 cells assessed as inhibition of cell proliferation measured after 5 days by ATPlite assay
Antiproliferative activity against human MV4-11 cells assessed as inhibition of cell proliferation measured after 5 days by ATPlite assay
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[PMID: 34283608] |
| MV4-11 | IC50 |
12.6 μM
Compound: 63; DDO-2213
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Antiproliferative activity against human MV4-11 cells assessed as cell viability incubated for 72 hrs by CCK8 assay
Antiproliferative activity against human MV4-11 cells assessed as cell viability incubated for 72 hrs by CCK8 assay
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[PMID: 34105966] |
Chemical Information
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CAS No. 2378768-36-4
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Appearance Solid
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Masse moléculaire 483.97
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Formule C24H27ClFN7O
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Color White to off-white
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SMILES
O=C(C1=CC=C(C2=CC(NC3=NC=NC(Cl)=C3N)=C(N4CCN(C)CC4)C=C2F)C=C1)N(C)C
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (206.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureté et documentation
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Fiche technique (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0662 mL | 10.3312 mL | 20.6624 mL | 51.6561 mL |
| 5 mM | 0.4132 mL | 2.0662 mL | 4.1325 mL | 10.3312 mL | |
| 10 mM | 0.2066 mL | 1.0331 mL | 2.0662 mL | 5.1656 mL | |
| 15 mM | 0.1377 mL | 0.6887 mL | 1.3775 mL | 3.4437 mL | |
| 20 mM | 0.1033 mL | 0.5166 mL | 1.0331 mL | 2.5828 mL | |
| 25 mM | 0.0826 mL | 0.4132 mL | 0.8265 mL | 2.0662 mL | |
| 30 mM | 0.0689 mL | 0.3444 mL | 0.6887 mL | 1.7219 mL | |
| 40 mM | 0.0517 mL | 0.2583 mL | 0.5166 mL | 1.2914 mL | |
| 50 mM | 0.0413 mL | 0.2066 mL | 0.4132 mL | 1.0331 mL | |
| 60 mM | 0.0344 mL | 0.1722 mL | 0.3444 mL | 0.8609 mL | |
| 80 mM | 0.0258 mL | 0.1291 mL | 0.2583 mL | 0.6457 mL | |
| 100 mM | 0.0207 mL | 0.1033 mL | 0.2066 mL | 0.5166 mL |
DDO-2213 Related Classifications
HY-153331 Related Classifications
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