Dodecylphosphocholine
Based on 1 Customer Validation
Dodecylphosphocholine is a detergent widely utilized in NMR studies of membrane proteins.
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- Pureté : 99.97%
- CAS No.: 29557-51-5
- Formule: C17H38NO4P
- Masse moléculaire:351.46
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
Dodecylphosphocholine micelles can be used to characterize pore formation the influenza M2 transmembrane (M2TM) domain by NMR spectroscopy[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 29557-51-5
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Appearance Solid
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Masse moléculaire 351.46
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Formule C17H38NO4P
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Color White to off-white
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SMILES
O=P([O-])(OCC[N+](C)(C)C)OCCCCCCCCCCCC
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
H2O : 100 mg/mL (284.53 mM; Need ultrasonic)
DMSO : 32.5 mg/mL (92.47 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.92 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.92 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Pureté et documentation
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Fiche technique (267 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. de Haas GH, Dijkman R, Ransac S, Verger R. Competitive inhibition of lipolytic enzymes. IV. Structural details of acylamino phospholipid analogues important for the potent inhibitory effects on pancreatic phospholipase A2. Biochim Biophys Acta. 1990;1046(3):249-257. [Content Brief]
[2]. François Dehez, et al. Mitochondrial ADP/ATP Carrier in Dodecylphosphocholine Binds Cardiolipins with Non-native Affinity. Biophys J. 2017 Dec 5;113(11):2311-2315. [Content Brief]
[3]. Sarah D Cady, et al. Specific binding of adamantane drugs and direction of their polar amines in the pore of the influenza M2 transmembrane domain in lipid bilayers and dodecylphosphocholine micelles determined by NMR spectroscopy. J Am Chem Soc. 2011 Mar 30;133(12):4274-84. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO / H2O | 1 mM | 2.8453 mL | 14.2264 mL | 28.4527 mL | 71.1319 mL |
| 5 mM | 0.5691 mL | 2.8453 mL | 5.6905 mL | 14.2264 mL | |
| 10 mM | 0.2845 mL | 1.4226 mL | 2.8453 mL | 7.1132 mL | |
| 15 mM | 0.1897 mL | 0.9484 mL | 1.8968 mL | 4.7421 mL | |
| 20 mM | 0.1423 mL | 0.7113 mL | 1.4226 mL | 3.5566 mL | |
| 25 mM | 0.1138 mL | 0.5691 mL | 1.1381 mL | 2.8453 mL | |
| 30 mM | 0.0948 mL | 0.4742 mL | 0.9484 mL | 2.3711 mL | |
| 40 mM | 0.0711 mL | 0.3557 mL | 0.7113 mL | 1.7783 mL | |
| 50 mM | 0.0569 mL | 0.2845 mL | 0.5691 mL | 1.4226 mL | |
| 60 mM | 0.0474 mL | 0.2371 mL | 0.4742 mL | 1.1855 mL | |
| 80 mM | 0.0356 mL | 0.1778 mL | 0.3557 mL | 0.8891 mL | |
| H2O | 100 mM | 0.0285 mL | 0.1423 mL | 0.2845 mL | 0.7113 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Dodecylphosphocholine Related Classifications
HY-116013 Related Classifications
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