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Cat. No.: HY-D1506

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Fl-DIBO

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Fl-DIBO (fluorogenic dibenzocyclooctyne) is a selective and high sensitivity fluorescent probe to azide compounds. Fl-DIBO can react rapidly with azide compounds to form new highly fluorescent products with a maximum emission wavelength of 469 nm and excitation wavelength of 363 nm. Fl-DIBO can be used to label diazo-tagged proteins without detectable background signal interference. Fl-DIBO is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

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Pureté: 99.60%
CAS No.: 1407523-31-2
Formule: C₁₉H₁₂O₃
Masse moléculaire:288.30

Stockage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Fl-DIBO Chemical Structure

CAS No.: 1407523-31-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- In Vitro
Chemical Information
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Fl-DIBO (fluorogenic dibenzocyclooctyne) is a selective and high sensitivity fluorescent probe to azide compounds. Fl-DIBO can react rapidly with azide compounds to form new highly fluorescent products with a maximum emission wavelength of 469 nm and excitation wavelength of 363 nm. Fl-DIBO can be used to label diazo-tagged proteins without detectable background signal interference^[1]^[2]. Fl-DIBO is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

In Vitro

Guide (The following is our recommended experimental protocol. This protocol is provided as a reference only and should be adjusted according to your specific needs).
Labeling of diazo-tagged proteins^[2]^[3]:
1. Resuspend the proteins in 1×PBS (pH 7.4), and incubate with NHS-activated ester (25 mM, dissolved in DMSO) overnight at room temperature.
2. Co-incubate the labeled proteins with Fl-DIBO (25-500 μM) at 37 °C for 18 h.
3. Analyze the proteins by SDS-PAGE gel electrophoresis and in-gel fluorescence imaging analysis, λ_ex=365 nm, λ_em=480 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 1407523-31-2
Appearance Solid
Masse moléculaire 288.30
Formule C₁₉H₁₂O₃
Color Light yellow to yellow
SMILES

O=C1C2=C1C3=CC=C(OC)C=C3C#CC4=C2C=CC(OC)=C4
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (267 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Ke Wang, et al. A metal-free turn-on fluorescent probe for the fast and sensitive detection of inorganic azides. Bioorg Med Chem Lett. 2016 Apr 1;26(7):1651-4. [Content Brief]

[2]. Frédéric Friscourt, et al. Fluorogenic Strain-Promoted Alkyne-Diazo Cycloadditions. Chemistry. 2015 Sep 28;21(40):13996-4001. doi: 10.1002/chem.201502242. Epub 2015 Aug 18. [Content Brief]

[3]. Camille Favre, et al. Sydnone Reporters for Highly Fluorogenic Copper-Free Click Ligations. J Org Chem. 2018 Feb 16;83(4):2058-2066. [Content Brief]

Calculators

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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