Chelidonine
Based on 2 publication(s) in Google Scholar
Chelidonine, an isoquinoline alkaloid, can be isolated from Chelidonium majus L.. Chelidonine causes G2/M arrest and induces caspase-dependent and caspase-independent apoptosis, and prevents cell cycle progression of stem cells in Dugesia japonica. Chelidonine has cytotoxic activity against melanoma cell lines. with anticancer and antiviral activity.
For research use only. We do not sell to patients.
- Purity: 99.91%
- CAS No.: 476-32-4
- Formula: C20H19NO5
- Molecular Weight:353.37
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Anti-Infection Compound Library
HY-L002
-
Apoptosis Compound Library
HY-L003
-
Natural Product Library
HY-L021
-
Anti-Cancer Compound Library
HY-L025
-
Antiviral Compound Library
HY-L027
-
Traditional Chinese Medicine Active Compound Library
HY-L065
-
Alkaloids Library
HY-L071
-
Anti-Cancer Natural Product Library
HY-L107
-
Antiviral Traditional Chinese Medicine Active Compound Library
HY-L113
-
Plant-Sourced Natural Product Library
HY-L115
-
Human Metabolite Library
HY-L123
-
Cell Death Library
HY-L162
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Natural Product Library Plus
HY-L021P
-
Natural Product and Natural Product-Like Compound Library
HY-L021M
-
Bioactive Compound Library
HY-L001
-
Miao Ethnicity Medicine Compound Library
HY-L190
-
Tibetan Medicine Compound Library
HY-L191
-
Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library
HY-L194
-
Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
-
RO5 Drug-like Natural Product Library
HY-L200
-
High-Throughput Bioactive Compound Library
HY-L205
-
High-Throughput Natural Product Library
HY-L206
-
Mass Spectrometry Human Metabolite Library
HY-L215
-
Antitussive and Antiasthmatic Traditional Chinese Medicine Active Compound Library
HY-L223
-
Diarrhea-Related Traditional Chinese Medicine Active Compound Library
HY-L224
-
Mongolian Medicine Compound Library
HY-L238
-
Natural Product Diversity Scaffold Library
HY-L245
-
Mass Spectrometry Natural Product Library
HY-L262
Publications Citing Use of MedChemExpress (MCE) Chelidonine
More
Biological Activity
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
>40 μM
Compound: 1
|
Antiproliferative activity against human HCT116 cells assessed as reduction in cell growth after 72 hrs by MTT assay
Antiproliferative activity against human HCT116 cells assessed as reduction in cell growth after 72 hrs by MTT assay
|
[PMID: 31883693] |
| HepG2 | IC50 |
>40 μM
Compound: 1
|
Antiproliferative activity against human HepG2 cells assessed as reduction in cell growth after 72 hrs by MTT assay
Antiproliferative activity against human HepG2 cells assessed as reduction in cell growth after 72 hrs by MTT assay
|
[PMID: 31883693] |
| HL-60 | IC50 |
2.2 μM
Compound: Chelidonine
|
Cytotoxicity against human HL-60 cells assessed as cell growth inhibition incubated for 48 hrs by XTT assay
Cytotoxicity against human HL-60 cells assessed as cell growth inhibition incubated for 48 hrs by XTT assay
|
[PMID: 34536668] |
| Jurkat | IC50 |
2.2 μM
Compound: Chelidonine
|
Cytotoxicity against human Jurkat cells assessed as cell growth inhibition incubated for 48 hrs by XTT assay
Cytotoxicity against human Jurkat cells assessed as cell growth inhibition incubated for 48 hrs by XTT assay
|
[PMID: 34536668] |
| K562 | IC50 |
17.65 μM
Compound: 1
|
Antiproliferative activity against human K562 cells assessed as reduction in cell growth after 72 hrs by MTT assay
Antiproliferative activity against human K562 cells assessed as reduction in cell growth after 72 hrs by MTT assay
|
[PMID: 31883693] |
| L02 | IC50 |
>40 μM
Compound: 1
|
Antiproliferative activity against human L02 cells assessed as reduction in cell growth after 72 hrs by MTT assay
Antiproliferative activity against human L02 cells assessed as reduction in cell growth after 72 hrs by MTT assay
|
[PMID: 31883693] |
| MCF7 | IC50 |
>40 μM
Compound: 1
|
Antiproliferative activity against human MCF7 cells assessed as reduction in cell growth after 72 hrs by MTT assay
Antiproliferative activity against human MCF7 cells assessed as reduction in cell growth after 72 hrs by MTT assay
|
[PMID: 31883693] |
| MOLT-4 | IC50 |
2.2 μM
Compound: Chelidonine
|
Cytotoxicity against human MOLT-4 cells assessed as cell growth inhibition incubated for 48 hrs by XTT assay
Cytotoxicity against human MOLT-4 cells assessed as cell growth inhibition incubated for 48 hrs by XTT assay
|
[PMID: 34536668] |
| PBMC | IC50 |
>40 μM
Compound: 1
|
Cytotoxicity against human PBMC assessed as reduction in cell growth after 72 hrs by MTT assay
Cytotoxicity against human PBMC assessed as reduction in cell growth after 72 hrs by MTT assay
|
[PMID: 31883693] |
| RAW264.7 | IC50 |
7.3 μM
Compound: 5
|
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by griess assay
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by griess assay
|
[PMID: 22024033] |
Chelidonine (5, 10 and 20 μM; 3-4 days) causes lesions and ventral curling in Dugesia japonica; and significantly decreases Djmcm2 expression at 20 μM but no reduction is observed at 5 and 10 μM; as well as prevents cell cycle progression of stem cells[2].
Chelidonine (0-3 μg/mL; 48 h) has cytotoxic activity against melanoma cell lines[3].
Chelidonine (1, 2 and 3 μg/mL; 24 h) decreases mitochondrial membrane potential (MMP) in 50% of A-375 cells at 1 and 1.5 μg/mL, and 62% at 3 μg/mL[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:A-375, A-375-p53DD and A-375-p53sh
-
Concentration:0-3 μg/mL
-
Incubation Time:48 h
-
Result:Exhibited cytotoxic activity against melanoma cell lines with 0.910±0.017 μg/ml, 0.634±0.009 μg/ml and 0.772±0.045 μg/ml in A-375, A-375-p53DD and A-375-p53sh, respectively.
Chemical Information
-
CAS No. 476-32-4
-
Appearance Solid
-
Molecular Weight 353.37
-
Formula C20H19NO5
-
Color White to off-white
-
SMILES
CN1[C@@]2([H])[C@@]([C@H](CC3=C2C=C4C(OCO4)=C3)O)([H])C5=C(C6=C(OCO6)C=C5)C1
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (2)
-
Journal Impact Factor
-
Most Recent
-
Antiviral Res
Discovery of hepatitis B virus subviral particle biogenesis inhibitors from a bioactive compound library. [Abstract]2024 Jul 2:228:105955. PMID: 38964614 -
Vet Microbiol
The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]2026 May:316:110992. PMID: 41865607
Solvent & Solubility
DMSO : 100 mg/mL (282.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (279 KB)
-
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
-
Handling Instructions (2659 KB)
References
[1]. Lee YK, et al. Chelidonine Induces Caspase-Dependent and Caspase-Independent Cell Death through G2/M Arrest in the T98G Human Glioblastoma Cell Line. Evid Based Complement Alternat Med. 2019 May 26;2019:6318179. [Content Brief]
[2]. Isolani ME, et al. The in vivo effect of chelidonine on the stem cell system of planarians. Eur J Pharmacol. 2012 Jul 5;686(1-3):1-7. [Content Brief]
[3]. Hammerová J, et al. Benzo[c]phenanthridine alkaloids exhibit strong anti-proliferative activity in malignant melanoma cells regardless of their p53 status. J Dermatol Sci. 2011 Apr;62(1):22-35. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8299 mL | 14.1495 mL | 28.2990 mL | 70.7474 mL |
| 5 mM | 0.5660 mL | 2.8299 mL | 5.6598 mL | 14.1495 mL | |
| 10 mM | 0.2830 mL | 1.4149 mL | 2.8299 mL | 7.0747 mL | |
| 15 mM | 0.1887 mL | 0.9433 mL | 1.8866 mL | 4.7165 mL | |
| 20 mM | 0.1415 mL | 0.7075 mL | 1.4149 mL | 3.5374 mL | |
| 25 mM | 0.1132 mL | 0.5660 mL | 1.1320 mL | 2.8299 mL | |
| 30 mM | 0.0943 mL | 0.4716 mL | 0.9433 mL | 2.3582 mL | |
| 40 mM | 0.0707 mL | 0.3537 mL | 0.7075 mL | 1.7687 mL | |
| 50 mM | 0.0566 mL | 0.2830 mL | 0.5660 mL | 1.4149 mL | |
| 60 mM | 0.0472 mL | 0.2358 mL | 0.4716 mL | 1.1791 mL | |
| 80 mM | 0.0354 mL | 0.1769 mL | 0.3537 mL | 0.8843 mL | |
| 100 mM | 0.0283 mL | 0.1415 mL | 0.2830 mL | 0.7075 mL |
Chelidonine Related Classifications
HY-N2369 Related Classifications
Powered by Bioz