CO probe 1
Based on 1 Customer Validation
CO probe 1 (probe 2) is a highly efficient fluorescent CO probe (Ex=493 nm) with an allyl ether reaction site. In the presence of PdCl₂, CO reduces Pd2+ to Pd0, triggering a Tsuji-Trost reaction that removes the allyl protecting group, releases fluorescein, and generates a significant fluorescence signal. CO probe 1 has high selectivity, rapid response (fluorescence enhancement of 150 times within 20 minutes), and low cytotoxicity, and can be used for real-time imaging of CO in living cells. CO probe 1 may be used to study pathological mechanisms involving CO signaling regulation, such as inflammation, vascular disease, or cancer.
For research use only. We do not sell to patients.
- Purity: 99.88%
- CAS No.: 1043865-37-7
- Formula: C26H18Cl2O5
- Molecular Weight:481.32
-
Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
CO probe 1 CO imaging steps in living cells[1]:
1. Before the cell imaging experiment, cells were seeded in 24-well culture plates overnight.
2. Set up control group and fluorescence group. Control group: Living cells were incubated with CO probe 1 (1 μM) at 37 °C for 30 minutes. Fluorescence group: Living cells were incubated with a mixture of CO probe 1 and PdCl2 (1 μM each) at 37 °C for 30 minutes.
3. After the above two groups were incubated, they were washed three times with PBS and then imaged.
* For monitoring exogenous CO, HeLa cells were pretreated with CORM-3 (HY-100581) (1, 5, and 10 μM, respectively) at 37 °C for 30 min and then incubated with a mixture of CO probe 1 and PdCl2 (1 μM each) at 37 °C for 30 min. Cells were washed with PBS buffer before imaging.
* For monitoring CO stimulated by heme, the procedure was essentially the same as for imaging exogenous CO, but heme (100 μM) was used instead of CORM 3 (HY-100581) in the cell pretreatment.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 1043865-37-7
-
Appearance Solid
-
Molecular Weight 481.32
-
Formula C26H18Cl2O5
-
Color Light yellow to yellow
-
SMILES
O=C1OC2(C3=CC(Cl)=C(C=C3OC4=CC(OCC=C)=C(C=C42)Cl)OCC=C)C5=C1C=CC=C5
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 25 mg/mL (51.94 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0776 mL | 10.3881 mL | 20.7762 mL | 51.9405 mL |
| 5 mM | 0.4155 mL | 2.0776 mL | 4.1552 mL | 10.3881 mL | |
| 10 mM | 0.2078 mL | 1.0388 mL | 2.0776 mL | 5.1940 mL | |
| 15 mM | 0.1385 mL | 0.6925 mL | 1.3851 mL | 3.4627 mL | |
| 20 mM | 0.1039 mL | 0.5194 mL | 1.0388 mL | 2.5970 mL | |
| 25 mM | 0.0831 mL | 0.4155 mL | 0.8310 mL | 2.0776 mL | |
| 30 mM | 0.0693 mL | 0.3463 mL | 0.6925 mL | 1.7313 mL | |
| 40 mM | 0.0519 mL | 0.2597 mL | 0.5194 mL | 1.2985 mL | |
| 50 mM | 0.0416 mL | 0.2078 mL | 0.4155 mL | 1.0388 mL |
Powered by Bioz