Cy3 hydrazide bromide
Based on 1 Customer Validation
Cy3 hydrazide bromide is a fluorescent probe for carbonylated proteins. Cy3 hydrazide bromide covalently links to aldehyde or ketone residues on carbonylated proteins, reacts with protein carbonyl groups to form stable hydrazones, and enables fluorescent detection (Ex/Em = 555/570 nm).
For research use only. We do not sell to patients.
- Formula: C31H41BrN4O7S2
- Molecular Weight:725.71
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Cy3 hydrazide bromide (30 min (on ice labeling)) labels carbonylated proteins in mouse latissimus dorsi muscle extracts[1].
Cy3 hydrazide bromide (5 mM; 30 min) enables multiplexed detection of carbonylated proteins in hydrogen peroxide-treated Saccharomyces cerevisiae W303-1A cell extracts via two-dimensional gel electrophoresis, but with spot shifting that reduces alignment with total protein stain[2].
Cy3 hydrazide bromide (0.5-40 mM; 45 min-2 h) labels yeast tRNAPhe at dihydrouridine residues[3].
Cy3 hydrazide bromide (40 mM; 2 h) labels reduced Escherichia coli tRNAArg[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 725.71
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Formula C31H41BrN4O7S2
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SMILES
O=C(NN)CCCCC[N+]1=C(C(C)(C2=C1C=CC(S(=O)(O)=O)=C2)C)/C=C/C=C3N(C4=CC=C(C=C4C\3(C)C)S(=O)(O)=O)CC.[Br-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 25 mg/mL (34.45 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 1.25 mg/mL (1.72 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
References
[1]. Nina-Diogo A, et al. Synthesis of New Bodipy Hydrazide Fluorescent Probes for the Detection of Carbonylated Proteins Generated by Oxidative Stress. Chembiochem. 2024;25(19):e202400093. [Content Brief]
[2]. Tamarit J, et al. Analysis of oxidative stress-induced protein carbonylation using fluorescent hydrazides. J Proteomics. 2012;75(12):3778-3788. [Content Brief]
[3]. Pan D, et al. Synthesis and functional activity of tRNAs labeled with fluorescent hydrazides in the D-loop. RNA. 2009;15(2):346-354. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.3780 mL | 6.8898 mL | 13.7796 mL | 34.4490 mL |
| DMSO | 5 mM | 0.2756 mL | 1.3780 mL | 2.7559 mL | 6.8898 mL |
| 10 mM | 0.1378 mL | 0.6890 mL | 1.3780 mL | 3.4449 mL | |
| 15 mM | 0.0919 mL | 0.4593 mL | 0.9186 mL | 2.2966 mL | |
| 20 mM | 0.0689 mL | 0.3445 mL | 0.6890 mL | 1.7225 mL | |
| 25 mM | 0.0551 mL | 0.2756 mL | 0.5512 mL | 1.3780 mL | |
| 30 mM | 0.0459 mL | 0.2297 mL | 0.4593 mL | 1.1483 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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