Cy3 hydrazide bromide

Cat. No.: HY-172285A: Data Sheet Handling Instructions
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Cy3 hydrazide bromide is a fluorescent probe for carbonylated proteins. Cy3 hydrazide bromide covalently links to aldehyde or ketone residues on carbonylated proteins, reacts with protein carbonyl groups to form stable hydrazones, and enables fluorescent detection (Ex/Em = 555/570 nm).

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Cy3 hydrazide bromide Chemical Structure

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5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
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Description	Cy3 hydrazide bromide is a fluorescent probe for carbonylated proteins. Cy3 hydrazide bromide covalently links to aldehyde or ketone residues on carbonylated proteins, reacts with protein carbonyl groups to form stable hydrazones, and enables fluorescent detection (Ex/Em = 555/570 nm)^[1]^[2]^[3].
In Vitro	Cy3 hydrazide bromide (30 min (on ice labeling)) labels carbonylated proteins in mouse latissimus dorsi muscle extracts^[1]. Cy3 hydrazide bromide (5 mM; 30 min) enables multiplexed detection of carbonylated proteins in hydrogen peroxide-treated Saccharomyces cerevisiae W303-1A cell extracts via two-dimensional gel electrophoresis, but with spot shifting that reduces alignment with total protein stain^[2]. Cy3 hydrazide bromide (0.5-40 mM; 45 min-2 h) labels yeast tRNA^Phe at dihydrouridine residues^[3]. Cy3 hydrazide bromide (40 mM; 2 h) labels reduced Escherichia coli tRNA^Arg^[3]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Cy3 hydrazide bromide Related Antibodies
Molecular Weight	725.71
Formula	C₃₁H₄₁BrN₄O₇S₂
SMILES	O=C(NN)CCCCC[N+]1=C(C(C)(C2=C1C=CC(S(=O)(O)=O)=C2)C)/C=C/C=C3N(C4=CC=C(C=C4C\3(C)C)S(=O)(O)=O)CC.[Br-]
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation	Data Sheet (273 KB) Handling Instructions (2659 KB)
References	[1]. Nina-Diogo A, et al. Synthesis of New Bodipy Hydrazide Fluorescent Probes for the Detection of Carbonylated Proteins Generated by Oxidative Stress. Chembiochem. 2024;25(19):e202400093. [2]. Tamarit J, et al. Analysis of oxidative stress-induced protein carbonylation using fluorescent hydrazides. J Proteomics. 2012;75(12):3778-3788. [3]. Pan D, et al. Synthesis and functional activity of tRNAs labeled with fluorescent hydrazides in the D-loop. RNA. 2009;15(2):346-354.