Cy3 hydrazide 

Cy3 hydrazide is a fluorescent probe for carbonylated proteins. Cy3 hydrazide covalently links to aldehyde or ketone residues on carbonylated proteins, reacts with protein carbonyl groups to form stable hydrazones, and enables fluorescent detection (Ex/Em = 555/570 nm)^[1][2][3].

Cy3 hydrazide (30 min (on ice labeling)) labels carbonylated proteins in mouse latissimus dorsi muscle extracts^[1].
Cy3 hydrazide (5 mM; 30 min) enables multiplexed detection of carbonylated proteins in hydrogen peroxide-treated Saccharomyces cerevisiae W303-1A cell extracts via two-dimensional gel electrophoresis, but with spot shifting that reduces alignment with total protein stain^[2].
Cy3 hydrazide (0.5-40 mM; 45 min-2 h) labels yeast tRNA^Phe at dihydrouridine residues^[3].
Cy3 hydrazide (40 mM; 2 h) labels reduced Escherichia coli tRNA^Arg[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

644.80

C₃₁H₄₀N₄O₇S₂

871514-32-8

O=C(CCCCC[N+]1=C(/C=C/C=C2N(CC)C3=CC=C(S(=O)(O)=O)C=C3C\2(C)C)C(C)(C)C4=C1C=CC(S(=O)([O-])=O)=C4)NN

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Nina-Diogo A, et al. Synthesis of New Bodipy Hydrazide Fluorescent Probes for the Detection of Carbonylated Proteins Generated by Oxidative Stress. Chembiochem. 2024;25(19):e202400093.

  [2]. Tamarit J, et al. Analysis of oxidative stress-induced protein carbonylation using fluorescent hydrazides. J Proteomics. 2012;75(12):3778-3788.

  [3]. Pan D, et al. Synthesis and functional activity of tRNAs labeled with fluorescent hydrazides in the D-loop. RNA. 2009;15(2):346-354.