Cy3 hydrazide
Cy3 hydrazide is a fluorescent probe for carbonylated proteins. Cy3 hydrazide covalently links to aldehyde or ketone residues on carbonylated proteins, reacts with protein carbonyl groups to form stable hydrazones, and enables fluorescent detection (Ex/Em = 555/570 nm).
For research use only. We do not sell to patients.
- CAS No.: 871514-32-8
- Formula: C31H40N4O7S2
- Molecular Weight:644.80
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Cy3 hydrazide (30 min (on ice labeling)) labels carbonylated proteins in mouse latissimus dorsi muscle extracts[1].
Cy3 hydrazide (5 mM; 30 min) enables multiplexed detection of carbonylated proteins in hydrogen peroxide-treated Saccharomyces cerevisiae W303-1A cell extracts via two-dimensional gel electrophoresis, but with spot shifting that reduces alignment with total protein stain[2].
Cy3 hydrazide (0.5-40 mM; 45 min-2 h) labels yeast tRNAPhe at dihydrouridine residues[3].
Cy3 hydrazide (40 mM; 2 h) labels reduced Escherichia coli tRNAArg[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 871514-32-8
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Molecular Weight 644.80
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Formula C31H40N4O7S2
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SMILES
O=C(CCCCC[N+]1=C(/C=C/C=C2N(CC)C3=CC=C(S(=O)(O)=O)C=C3C\2(C)C)C(C)(C)C4=C1C=CC(S(=O)([O-])=O)=C4)NN
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Nina-Diogo A, et al. Synthesis of New Bodipy Hydrazide Fluorescent Probes for the Detection of Carbonylated Proteins Generated by Oxidative Stress. Chembiochem. 2024;25(19):e202400093. [Content Brief]
[2]. Tamarit J, et al. Analysis of oxidative stress-induced protein carbonylation using fluorescent hydrazides. J Proteomics. 2012;75(12):3778-3788. [Content Brief]
[3]. Pan D, et al. Synthesis and functional activity of tRNAs labeled with fluorescent hydrazides in the D-loop. RNA. 2009;15(2):346-354. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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