2. Metabolic Enzyme/Protease Autophagy Apoptosis PI3K/Akt/mTOR MAPK/ERK Pathway Stem Cell/Wnt
  3. NAMPT Autophagy Apoptosis mTOR p38 MAPK ERK
  4. Daporinad hydrochloride

Daporinad hydrochloride  (Synonyms: FK866 hydrochloride; APO866 hydrochloride)

Cat. No.: HY-50876A
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Daporinad (FK866) hydrochloride is a non-competitive inhibitor of nicotinamide phosphoribosyltransferase (Nampt), with a Ki value of 0.3 nM. Daporinad hydrochloride depletes NAD+ and ATP levels, inhibits mTORC1 and MAPK/ERK pathways, and activates TFEB to induce autophagy. Daporinad hydrochloride causes the depletion of the endoplasmic reticulum Ca²⁺ pool, ultimately weakening the mitogen-induced Ca²⁺ signal and the activation and function of T cells. Daporinad hydrochloride induces cell cycle arrest and apoptosis, and inhibits cell proliferation. Daporinad hydrochloride can be used for the study of myeloma, liver cancer, and immunosuppression.

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CAS No. : 2727965-45-7

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64 Publications Citing Use of MCE Daporinad hydrochloride

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    Daporinad hydrochloride purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Dec 29:S2090-1232(25)01020-3.  [Abstract]

    Representative ocular fundus images and clinical scores from the Normal, EAU, and NAMPTi (FK866; 10 mg/kg; ip; daily for 14 days ) groups at day 14 post-immunization. White arrows highlight inflammatory infiltrates and vascular abnormalities. B. Representative histopathological images and histological scores from the Normal, EAU and NAMPTi groups at day 14 post-immunization. Black arrows highlight inflammatory cell infiltration, retinal detachment, and retinal folding.

    Daporinad hydrochloride purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Dec 29:S2090-1232(25)01020-3.  [Abstract]

    NAMPT inhibition ameliorates EAU by modulating the Teff/Treg balance and suppressing Hif1a in CD4+ T cells in vivo. A-B. Frequencies of CD4+IL 17A+ (Th17) cells and CD4+ IFN γ+(Th1) cells in both CDLNs (A) and spleen (B) of EAU and NAMPTi (FK866; 10 mg/kg; ip; daily for 14 days ) treated EAU mice on day 14 post-immunization. C-D. Frequencies of CD4+-CD25+Foxp3+ (Treg) cells in both CDLNs (C) and spleen (D).

    Daporinad hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Metab. 2024 Jun 18:S1550-4131(24)00189-X.  [Abstract]

    Flow cytometry analysis of M1 (CD86) and M2 (CD206) markers in human peripheral blood-derived macrophages or NAMPT-overexpressing (oxNAMPT) macrophages both treated with FK866 (10 nM, 48 h).

    Daporinad hydrochloride purchased from MedChemExpress. Usage Cited in: J Cell Physiol. 2019 Apr;234(4):4385-4395.  [Abstract]

    SW620 and HCT116 cells are seeded into a six-well plate for 72 hr, followed by treatment with different concentrations (100 nM and 10 μM) of FK866 for 24 hr.

    Daporinad hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2017 Oct;16(4):5121-5128.  [Abstract]

    Effects of Emodin, FK866 and DEX on PMN apoptosis in SAP rats. Protein expression levels of Fas, FasL, Bax, cleaved caspase 3 and Bcl xL are detected by western blotting. β actin served as an internal control. Data are presented as the mean±standard deviation.

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    Description

    Daporinad (FK866) hydrochloride is a non-competitive inhibitor of nicotinamide phosphoribosyltransferase (Nampt), with a Ki value of 0.3 nM. Daporinad hydrochloride depletes NAD+ and ATP levels, inhibits mTORC1 and MAPK/ERK pathways, and activates TFEB to induce autophagy. Daporinad hydrochloride causes the depletion of the endoplasmic reticulum Ca²⁺ pool, ultimately weakening the mitogen-induced Ca²⁺ signal and the activation and function of T cells. Daporinad hydrochloride induces cell cycle arrest and apoptosis, and inhibits cell proliferation. Daporinad hydrochloride can be used for the study of myeloma, liver cancer, and immunosuppression[1][2][3][4].

    Molecular Weight

    427.97

    Formula

    C24H30ClN3O2
    CAS No.
    SMILES

    O=C(C1=CC=CC=C1)N2CCC(CC2)CCCCNC(/C=C/C3=CN=CC=C3)=O.Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
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    Daporinad hydrochloride Related Classifications

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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Daporinad2727965-45-7FK866APO866FK 866FK-866APO 866APO-866NAMPTAutophagyApoptosismTORp38 MAPKERKNicotinamide phosphoribosyl transferasePBEFVisfatinpre-B cell-enhancing factorPre-B cell colony enhancing factorMammalian target of RapamycinExtracellular signal regulated kinasesNAMPT inhibitorNoncompetitive inhibitionNAD+ depletionDelayed cell deathEnzyme kineticsNicotinamideCancer therapy.Inhibitorinhibitorinhibit

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