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  3. Fluorescent Dye
  4. DCVJ

DCVJ  (Synonyms: 9-(2,2-Dicyanovinyl)julolidine)

Cat. No.: HY-D1425 Purity: 99.80%
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DCVJ (9-(2,2-Dicyanovinyl)julolidine), a molecular rotor and unique fluorescent dye, binds to tubulin and actin, and increases its fluorescence intensity drastically upon polymerization. DCVJ also binds to phospholipid bilayers and increases its fluorescence intensity. DCVJ can detect the kinetic process of degranulation of mast cells.

For research use only. We do not sell to patients.

DCVJ Chemical Structure

DCVJ Chemical Structure

CAS No. : 58293-56-4

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10 mg USD 100 In-stock
25 mg USD 200 In-stock
50 mg USD 300 In-stock
100 mg USD 450 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

DCVJ (9-(2,2-Dicyanovinyl)julolidine), a molecular rotor and unique fluorescent dye, binds to tubulin and actin, and increases its fluorescence intensity drastically upon polymerization. DCVJ also binds to phospholipid bilayers and increases its fluorescence intensity. DCVJ can detect the kinetic process of degranulation of mast cells[1].

Molecular Weight

249.31

Formula

C16H15N3
CAS No.
Appearance

Solid

Color

Light brown to brown

SMILES

N#C/C(C#N)=C/C1=CC(CCCN2CCC3)=C2C3=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (250.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.0111 mL 20.0554 mL 40.1107 mL
5 mM 0.8022 mL 4.0111 mL 8.0221 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.0111 mL 20.0554 mL 40.1107 mL 100.2768 mL
5 mM 0.8022 mL 4.0111 mL 8.0221 mL 20.0554 mL
10 mM 0.4011 mL 2.0055 mL 4.0111 mL 10.0277 mL
15 mM 0.2674 mL 1.3370 mL 2.6740 mL 6.6851 mL
20 mM 0.2006 mL 1.0028 mL 2.0055 mL 5.0138 mL
25 mM 0.1604 mL 0.8022 mL 1.6044 mL 4.0111 mL
30 mM 0.1337 mL 0.6685 mL 1.3370 mL 3.3426 mL
40 mM 0.1003 mL 0.5014 mL 1.0028 mL 2.5069 mL
50 mM 0.0802 mL 0.4011 mL 0.8022 mL 2.0055 mL
60 mM 0.0669 mL 0.3343 mL 0.6685 mL 1.6713 mL
80 mM 0.0501 mL 0.2507 mL 0.5014 mL 1.2535 mL
100 mM 0.0401 mL 0.2006 mL 0.4011 mL 1.0028 mL
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DCVJ Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DCVJ58293-56-49-(2,2-Dicyanovinyl)julolidineFluorescent Dyefluorescent dyetubulinactinpolymerizationphospholipid bilayersdegranulationmast cellsInhibitorinhibitorinhibit

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