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Art. -Nr.: HY-D1024

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Coelenterazine h

(Synonyms: 2-Deoxycoelenterazine; CLZN-h)

3 Cited Publications

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Based on 3 publication(s) in Google Scholar

Coelenterazine h (2-Deoxycoelenterazine), a coelenterazine derivative, is a luminescent substrate for RLuc8. Coelenterazine h is more sensitive to Ca²⁺, thus providing a valuable tool for measuring small changes in Ca²⁺ concentrations (Ex/Em = 437/466 nm).

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Reinheit: 99.41%
CAS. Nr.: 50909-86-9
Formel: C₂₆H₂₁N₃O₂
Molecular Weight:407.46

Speicherung:

-20°C, sealed storage, away from moisture and light

* The compound is unstable in solutions, freshly prepared is recommended.

Coelenterazine h Chemical Structure

CAS. Nr.: 50909-86-9

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Publications (3)
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle Calcium Channel Isoform-spezifische Produkte anzeigen :

In Vitro

Coelenterazine h (at concentrations of 1-10 μM) can be used as the luminescent substrate for RLuc8 [4].
In the Ca²⁺ binding kinetics measurement of BRAC, 5 nM BRAC protein was rapidly mixed with 20 μM Coelenterazine h in different concentrations of Ca²⁺ buffer solution, and the Venus (530 nm) emission intensity of BRAC was monitored at 1 kHz [4].
Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
1. Solution Preparation^[4]
1.1 Preparation of Stock Solution
Solvent: Methanol or Ethanol
Concentration: 10 mM (optimized according to the experiment).
1.2 Solution Preparation
Dilute to 10 μM using PBS or cell culture medium (based on experimental optimization).
Note: The raw solution and working solution should be prepared and used immediately. Store them in a dark place.

2. Cell Staining
2.1 Transfect Hela cells with BRAC or G5A.
2.2 Add 10 μM Coelenterazine h to the cell plate.
2.3 Add the dye and the template to the cell culture box and incubate for 1 to 4 hours (depending on the experimental optimization).
2.4 Fluorescence microscopy detection (Ex/Em = 437/466 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 50909-86-9
Appearance Solid
Molecular Weight 407.46
Formel C₂₆H₂₁N₃O₂
Color Yellow to orange
SMILES

OC(C=C1)=CC=C1C2=CN(C(C(CC3=CC=CC=C3)=N4)=O)C4=C(N2)CC5=CC=CC=C5
Synonyms

2-Deoxycoelenterazine; CLZN-h
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, sealed storage, away from moisture and light

* The compound is unstable in solutions, freshly prepared is recommended.

Publications (3)

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Stress Biol

A self-assembling split Nano luciferase-based assay for investigating Pseudomonas syringae effector secretion. [Abstract]

2024 Feb 16;4(1):14. PMID: 38363371

Coelenterazine h purchased from MedChemExpress. Usage Cited in: Stress Biol. 2024 Feb 16;4(1):14. [Abstract]

A. tumefaciens strains carrying NlucN-FLAG, along with avrRpt2-HA-HiBiT or avrPto-HA-HiBiT, were infiltrated into the leaves. Thirty hours after infiltration, leaves discs were collected and incubated with Coelenterazine h (10 µM; 10 min) in a 96-well plate, then the luminescence was monitored using EnSpire Multimode Plate Reader.

Coelenterazine h purchased from MedChemExpress. Usage Cited in: Stress Biol. 2024 Feb 16;4(1):14. [Abstract]

Leaves of transgenic A. thaliana plants expressing NlucN were infiltrated with H2O or 1 μM flg22. 24 hours after first infiltration, indicated bacteria (OD600 = 0.1) was infiltrated to the same leaves. Leaf discs were harvested 9 h later, and incubated with Coelenterazine h (10 µM; 10 min) in a 96-well plate, then the luminescence was monitored using EnSpire Multimode Plate Reader.

Coelenterazine h purchased from MedChemExpress. Usage Cited in: Stress Biol. 2024 Feb 16;4(1):14. [Abstract]

Effector secretion was induced in vitro. Culture supernatants were collected, incubated with Coelenterazine h (10 μM; 10 min) and NlucN-His, and measured for luminescence. Total protein was analyzed by immunoblot using anti-AvrPto and anti-HA antibodies to detect the accumulation of AvrPto-HiBiT and AvrRpt2-HA-HiBiT. Signal-to-noise ratio was shown.
J Biol Chem

Eyeless razor clam Sinonovacula constricta discriminates light spectra through opsins to guide Ca2+ and cAMP signaling pathways. [Abstract]

2024 Jan;300(1):105527. PMID: 38043801

Coelenterazine h purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2024 Jan;300(1):105527. [Abstract]

Coelenterazine h (10 μM; 2 h). HEK293T cells transfected with the empty vector (no opsin) served as the negative control, while those transfected with H. sapiens Opn4 were used as the positive control to measure the luminescence of Gαq-mediated Ca²⁺ increase.
ACS Pharmacol Transl Sci

Rational Design Biased Compounds against μ‑Opioid Receptor. [Abstract]

2025 Apr 8;8(7):1996-2008. PMID: 40672676

Coelenterazine h purchased from MedChemExpress. Usage Cited in: ACS Pharmacol Transl Sci. 2025 Apr 8;8(7):1996-2008. [Abstract]

Coelenterazine h (5 μM; 5 min). Concentration-dependent inhibition of forskolin-induced (5 μM) cAMP accumulation using a BRET-Epac biosensor.

Reinheit & Dokumentation

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Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Jiang T, et al. New bioluminescent coelenterazine derivatives with various C-6 substitutions. Org Biomol Chem. 2017 Aug 23;15(33):7008-7018. [Content Brief]

[2]. M R Knight, et al. Imaging calcium dynamics in living plants using semi-synthetic recombinant aequorins. J Cell Biol. 1993 Apr;121(1):83-90. [Content Brief]

[3]. Kazushi Suzuki, et al. Five colour variants of bright luminescent protein for real-time multicolour bioimaging. Nat Commun. 2016 Dec 14:7:13718. [Content Brief]

[4]. Kenta Saito, et al. Auto-luminescent genetically-encoded ratiometric indicator for real-time Ca2+ imaging at the single cell level. PLoS One. 2010 Apr 1;5(4):e9935. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Coelenterazine h

3 Cited Publications

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Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Publications (3)
Reinheit & Dokumentation
Verweise

1/16
1/16

Coelenterazine h purchased from MedChemExpress. Usage Cited in: ACS Pharmacol Transl Sci. 2025 Apr 8;8(7):1996-2008. [Abstract]

Customer Validation
Coelenterazine h (5 μM; 5 min). Concentration-dependent inhibition of forskolin-induced (5 μM) cAMP accumulation using a BRET-Epac biosensor.
1/16

Coelenterazine h purchased from MedChemExpress. Usage Cited in: Stress Biol. 2024 Feb 16;4(1):14. [Abstract]

Customer Validation
A. tumefaciens strains carrying NlucN-FLAG, along with avrRpt2-HA-HiBiT or avrPto-HA-HiBiT, were infiltrated into the leaves. Thirty hours after infiltration, leaves discs were collected and incubated with Coelenterazine h (10 µM; 10 min) in a 96-well plate, then the luminescence was monitored using EnSpire Multimode Plate Reader.
1/16

Coelenterazine h purchased from MedChemExpress. Usage Cited in: Stress Biol. 2024 Feb 16;4(1):14. [Abstract]

Customer Validation
Leaves of transgenic A. thaliana plants expressing NlucN were infiltrated with H2O or 1 μM flg22. 24 hours after first infiltration, indicated bacteria (OD600 = 0.1) was infiltrated to the same leaves. Leaf discs were harvested 9 h later, and incubated with Coelenterazine h (10 µM; 10 min) in a 96-well plate, then the luminescence was monitored using EnSpire Multimode Plate Reader.
1/16

Coelenterazine h purchased from MedChemExpress. Usage Cited in: Stress Biol. 2024 Feb 16;4(1):14. [Abstract]

Customer Validation
Effector secretion was induced in vitro. Culture supernatants were collected, incubated with Coelenterazine h (10 μM; 10 min) and NlucN-His, and measured for luminescence. Total protein was analyzed by immunoblot using anti-AvrPto and anti-HA antibodies to detect the accumulation of AvrPto-HiBiT and AvrRpt2-HA-HiBiT. Signal-to-noise ratio was shown.
1/16

Coelenterazine h purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2024 Jan;300(1):105527. [Abstract]

Customer Validation
Coelenterazine h (10 μM; 2 h). HEK293T cells transfected with the empty vector (no opsin) served as the negative control, while those transfected with H. sapiens Opn4 were used as the positive control to measure the luminescence of Gαq-mediated Ca²⁺ increase.

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Coelenterazine h

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Publications Citing Use of MedChemExpress (MCE) Coelenterazine h

Alle Calcium Channel Isoform-spezifische Produkte anzeigen

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Chemical Information

Publications (3)

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Coelenterazine h

MCE Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) Coelenterazine h

Alle Calcium Channel Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (3)

Reinheit & Dokumentation

Verweise

Calculators

Help & FAQs

Coelenterazine h Related Classifications

HY-D1024 Related Classifications