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Art. -Nr.: HY-183406

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AS15

AS15 is a PDIA1 inhibitor with an IC₅₀ value of 300 nM. AS15 interacts with the active-site cysteine of PDIA1, and this process is independent of the substrate-binding domain of PDIA1. AS15 acts as a synergistic cell growth inhibitor with buthionine sulfoximine (BSO) (HY-106376), a glutathione synthesis inhibitor. AS15 can be used in the research of glioblastoma.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

CAS. Nr.: 102616-62-6
Formel: C₂₀H₂₃NO₆
Molecular Weight:373.40

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

AS15 Chemical Structure

CAS. Nr.: 102616-62-6

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

^[1]

PDIA1

300 nM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
Calu-6	IC₅₀	20 μM Compound: 15, NSC368252	Inhibition of human His-tagged Frataxin/GST-tagged mono ubiquitin interaction expressed in human Calu6 cells after 3 days by GST pull down-based Western blot analysis Inhibition of human His-tagged Frataxin/GST-tagged mono ubiquitin interaction expressed in human Calu6 cells after 3 days by GST pull down-based Western blot analysis	[PMID: 23506486]
U-87MG ATCC	IC₅₀	18.3 μM Compound: AS15/NC014; NSC 368252	Cytotoxicity against human U-87 MG cells assessed as growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against human U-87 MG cells assessed as growth inhibition measured after 72 hrs by MTT assay	[PMID: 32830969]

In Vitro

Anti-cancer

AS15 (0.3-3 μM; 24 h) reduces the colony-forming ability of U-118 MG glioblastoma cells, and its activity can be enhanced by glutathione depletion via pre-treatment with BSO^[1].
AS15 reduces the viability of U-87 MG glioblastoma cells, with an IC₅₀ of 18.3 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

U-118 MG glioblastoma cells
Concentration:

0.3, 1 and 3 μM
Incubation Time:

24 h (BSO pretreatment)
Result:

Increased potency in reducing colony formation after pretreatment with BSO (a glutathione synthesis inhibitor), supporting that glutathione depletion sensitizes glioblastoma cells to AS15.

Related Protocols :

Chemical Information

CAS. Nr. 102616-62-6
Molecular Weight 373.40
Formel C₂₀H₂₃NO₆
SMILES

OC1=C(C(N2CCOCC2)C3=CC=C(C(OC)=C3)OC)C=C4OCOC4=C1
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Handling Instructions (2659 KB)

Verweise

[1]. Shergalis A, et al. Characterization of Aminobenzylphenols as Protein Disulfide Isomerase Inhibitors in Glioblastoma Cell Lines. Journal of medicinal chemistry. 2020 Sep 24;63(18):10263-10286. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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