Pyraflufen-ethyl
Based on 1 Customer Validation
Pyraflufen-ethyl is a phenylpyrazole herbicide for control of annual and perennial broadleaf weeds. Pyraflufen-ethyl inhibits protoporphyrinogen oxidase (PPO). Pyraflufen-ethyl controls rosette Parthenium hysterophorus.
For research use only. We do not sell to patients.
- Purity: 98.02%
- CAS No.: 129630-19-9
- Formula: C15H13Cl2F3N2O4
- Molecular Weight:413.18
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Biological Activity
Pyraflufen-ethyl (2.33-2.65 g/AI; 14 days) reduces aboveground dry weight of rosette Parthenium hysterophorus[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 129630-19-9
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Appearance Solid
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Molecular Weight 413.18
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Formula C15H13Cl2F3N2O4
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Color Light yellow to light brown
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SMILES
O=C(OCC)COC1=CC(C2=NN(C)C(OC(F)F)=C2Cl)=C(F)C=C1Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (242.03 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (3.03 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (458 KB)
- English - EN (458 KB)
- Français - FR (458 KB)
- Deutsch - DE (458 KB)
- Norwegian - NO (458 KB)
- Español - ES (458 KB)
- Swedish - SV (458 KB)
- Italian - IT (458 KB)
- Korean - KR (458 KB)
- Portuguese - PT (458 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4203 mL | 12.1013 mL | 24.2025 mL | 60.5063 mL |
| 5 mM | 0.4841 mL | 2.4203 mL | 4.8405 mL | 12.1013 mL | |
| 10 mM | 0.2420 mL | 1.2101 mL | 2.4203 mL | 6.0506 mL | |
| 15 mM | 0.1614 mL | 0.8068 mL | 1.6135 mL | 4.0338 mL | |
| 20 mM | 0.1210 mL | 0.6051 mL | 1.2101 mL | 3.0253 mL | |
| 25 mM | 0.0968 mL | 0.4841 mL | 0.9681 mL | 2.4203 mL | |
| 30 mM | 0.0807 mL | 0.4034 mL | 0.8068 mL | 2.0169 mL | |
| 40 mM | 0.0605 mL | 0.3025 mL | 0.6051 mL | 1.5127 mL | |
| 50 mM | 0.0484 mL | 0.2420 mL | 0.4841 mL | 1.2101 mL | |
| 60 mM | 0.0403 mL | 0.2017 mL | 0.4034 mL | 1.0084 mL | |
| 80 mM | 0.0303 mL | 0.1513 mL | 0.3025 mL | 0.7563 mL | |
| 100 mM | 0.0242 mL | 0.1210 mL | 0.2420 mL | 0.6051 mL |