Dextran sucrase

Dextran sucrase catalyzes glucosyl transfer using sucrose to synthesize dextran, and the K_m value of purified free Dextran sucrase for sucrose is 0.018 M^[2].
After adding 0.05% CaCl₂ to the purified enzyme preparation, the activity of Dextran sucrase increases from 12.6 DSU/mL to 33.9 DSU/mL, and the activity recovery rate rises from approximately 33% to 86%^[2].
When dextran sucrase is immobilized on glutaraldehyde-activated porous silica, the activity of the immobilized enzyme reaches 830 DSU/g support when a carrier with a specific surface area of 6 m²/g is used and maltose is added during the immobilization process^[2] .
Dextran sucrase (sucrose: 10%; 30°C) can synthesize dextran with a molecular weight greater than 10⁶. Under the same reaction conditions, the molecular weight of dextran produced by immobilized Dextran sucrase is higher than that produced by purified free enzyme^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.