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Cat. No.: HY-DY1111

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DFHBI-2T (solution)

DFHBI-2T solution is a membrane-permeable, RNA aptamer-activated fluorescent probe (Ex/Em=500 nm/523 nm), with a K_d value of 1300 nM for the RNA aptamer Spinach2. DFHBI-2T solution binds to Spinach2, converting the fluorophore to a fluorescent state, stabilizing its planar structure to facilitate the radiative fluorescence decay pathway, and forming a complex with red-shifted excitation and emission peaks. DFHBI-2T solution can be used for RNA imaging in live cells. It is also applicable to research related to fragile X-associated tremor/ataxia syndrome.

For research use only. We do not sell to patients.

CAS No.: 1539318-40-5
Formula: C₁₂H₇F₅N₂O₂
Molecular Weight:306.19

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

DFHBI-2T (solution) Chemical Structure

CAS No.: 1539318-40-5

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
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Biological Activity

Description

In Vitro

DFHBI-2T solution (1 μM) binds Spinach2 RNA aptamer with a K_d of 1300 nM, forming a complex with excitation maximum at 500 nm, emission maximum at 523 nm^[1].
DFHBI-2T solution (20 μM; 10 min) forms a fluorescent complex that is readily detectable using a YFP filter cube but not a GFP filter cube in COS7 cells expressing (CGG)₆₀-Spinach2^[1].
DFHBI-2T solution enables SAM-dependent fluorescence detection at 523 nm using a Spinach2-based SAM sensor in vitro^[1].
DFHBI-2T solution binds to the Spinach2 RNA aptamer with a K_d of 1300 nM, exhibiting a shift in spectral properties and a 60-fold increase in brightness compared to its free state^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 1539318-40-5
Molecular Weight 306.19
Formula C₁₂H₇F₅N₂O₂
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Song W, et al. Plug-and-play fluorophores extend the spectral properties of Spinach. Journal of the American Chemical Society. 2014 Jan 29;136(4):1198-201. [Content Brief]

[2]. Ouellet J, et al. RNA Fluorescence with Light-Up Aptamers. Frontiers in chemistry. 2016;4:29.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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