Gap 26
Based on 9 publication(s) in Google Scholar
Gap 26 is a connexin mimetic peptide, composed of residue numbers 63-75 of the first extracellular loop of connexin 43 (gap junction blocker), containing the SHVR amino acid motif.
For research use only. We do not sell to patients.
- Purity: 99.98%
- CAS No.: 197250-15-0
- Formula: C70H107N19O19S
- Molecular Weight:1550.78
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Storage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Gap 26
More- Cell Metab. 2021 Feb 2;33(2):283-299.e9. [Abstract]
- J Ethnopharmacol. 2026 Nov 15:370:122013. [Abstract]
- Toxicology. 2022 Aug:478:153283. [Abstract]
- Lab Invest. 2022 Jan;102(1):90-101. [Abstract]
- J Immunol. 2025 Jan 1;214(1):130-140. [Abstract]
- ACS Chem Biol. 2020 Jun 19;15(6):1392-1400. [Abstract]
- Medicina (Kaunas). 2024 May 8;60(5):780. [Abstract]
- Cureus. 2024 Apr 26;16(4):e59053. [Abstract]
- Biomed Pharmacother. 2022 Apr 22;150:112973. [Abstract]
Biological Activity
Gap Junction Protein[1]
Gap 26 (0.25 mg/mL, 30 min) reduces the wave size in the three cell lines (RBE4, SV-ARBEC and ECV304). Gap 26 (0.25 mg/mL, 30 min) completely abolishes the InsP3-triggered ATP response and reduced the ATP release to below the control level, indicating that the basal ATP release is also affected[1].
Gap 26 does indeed significantly inhibit our InsP3-triggered intercellular calcium waves, but it did not have any effect on dye coupling through junctional channels as evidenced by the FRAP experiments, despite the fact that connexin 43 was present in the cell lines used[1].
Gap 26 (100-300 μM) dose-dependently reduces the rhythmic responses of rabbit superior mesenteric arteries, with IC50 of 28.4 ± 3.4 μM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 197250-15-0
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Appearance Solid
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Molecular Weight 1550.78
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Formula C70H107N19O19S
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Color White to off-white
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Sequence
Val-Cys-Tyr-Asp-Lys-Ser-Phe-Pro-Ile-Ser-His-Val-Arg
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Sequence Shortening
VCYDKSFPISHVR
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (9)
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Journal Impact Factor
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Most Recent
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Cell Metab
Platelets Facilitate the Wound-Healing Capability of Mesenchymal Stem Cells by Mitochondrial Transfer and Metabolic Reprogramming. [Abstract]2021 Feb 2;33(2):283-299.e9. PMID: 33400911 -
J Ethnopharmacol
Qishen paste improves cardiac conduction in heart failure by regulating cardiac resident macrophage Connexin43. [Abstract]2026 Nov 15:370:122013. PMID: 42269798 -
Toxicology
Oxidative stress and Cx43-mediated apoptosis are involved in PFOS-induced nephrotoxicity. [Abstract]2022 Aug:478:153283. PMID: 35934180 -
Lab Invest
Bioglass promotes wound healing by inhibiting endothelial cell pyroptosis through regulation of the connexin 43/reactive oxygen species (ROS) signaling pathway. [Abstract]2022 Jan;102(1):90-101. PMID: 34521991 -
J Immunol
Plasmacytoid dendritic cell sensing of African swine fever virus-infected macrophages results in STING-dependent robust interferon-α production. [Abstract]2025 Jan 1;214(1):130-140. PMID: 40073264 -
ACS Chem Biol
Cyanopyrrolidine Inhibitors of Ubiquitin Specific Protease 7 Mediate Desulfhydration of the Active-Site Cysteine. [Abstract]2020 Jun 19;15(6):1392-1400. PMID: 32302100 -
Medicina (Kaunas)
Comprehensive Pan-Cancer Analysis of Connexin 43 as a Potential Biomarker and Therapeutic Target in Human Kidney Renal Clear Cell Carcinoma (KIRC). [Abstract]2024 May 8;60(5):780. PMID: 38792963 -
Cureus
2024 Apr 26;16(4):e59053. PMID: 38680825 -
Biomed Pharmacother
Dioscin potentiates the antitumor effect of suicide gene therapy in melanoma by gap junction intercellular communication-mediated antigen cross-presentation. [Abstract]2022 Apr 22;150:112973. PMID: 35468581
Solvent & Solubility
H2O : 50 mg/mL (32.24 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (64.48 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (273 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Katleen Braet, et al. Photoliberating inositol-1,4,5-trisphosphate triggers ATP release that is blocked by the connexin mimetic peptide gap 26. Cell Calcium. 2003 Jan;33(1):37-48. [Content Brief]
[2]. Chaytor AT, et al. Peptides homologous to extracellular loop motifs of connexin 43 reversibly abolish rhythmic contractile activity in rabbit arteries. J Physiol. 1997 Aug 15;503 ( Pt 1):99-110. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.6448 mL | 3.2242 mL | 6.4484 mL | 16.1209 mL |
| 5 mM | 0.1290 mL | 0.6448 mL | 1.2897 mL | 3.2242 mL | |
| 10 mM | 0.0645 mL | 0.3224 mL | 0.6448 mL | 1.6121 mL | |
| 15 mM | 0.0430 mL | 0.2149 mL | 0.4299 mL | 1.0747 mL | |
| 20 mM | 0.0322 mL | 0.1612 mL | 0.3224 mL | 0.8060 mL | |
| 25 mM | 0.0258 mL | 0.1290 mL | 0.2579 mL | 0.6448 mL | |
| 30 mM | 0.0215 mL | 0.1075 mL | 0.2149 mL | 0.5374 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Gap 26 Related Classifications
HY-P1082 Related Classifications
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