PYCARD - PYD and CARD domain containing Gene

Also Known as ASC; TMS; TMS1; CARD5; TMS-1

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 29108

About PYCARD

Cytogenetic location: 16p11.2 Genomic coordinates (GRCh38): 16:31,201,486-31,202,760 (from NCBI)

This gene has 4 transcripts (splice variants), 139 orthologues and 16 paralogues. Broad expression in spleen (RPKM 25.5), colon (RPKM 25.4) and 22 other tissues.

Summary

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of Caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing Apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

PYCARD Products (2)

mRNA	Protein	Name
NM_013258.5	NP_037390.2	apoptosis-associated speck-like protein containing a CARD isoform a
NM_145182.3	NP_660183.1	apoptosis-associated speck-like protein containing a CARD isoform b

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables BMP receptor binding	IPI IPI: Inferred from physical interaction	24407287	GOA
enables Pyrin domain binding	IPI IPI: Inferred from physical interaction	15030775	GOA
enables enzyme binding	IPI IPI: Inferred from physical interaction	24407287	GOA
enables identical protein binding	IDA IDA: Inferred from direct assay	34341353	GOA
enables identical protein binding	IPI IPI: Inferred from physical interaction	24630722	GOA
enables interleukin-6 receptor binding	IPI IPI: Inferred from physical interaction	24407287	GOA
enables myosin I binding	IPI IPI: Inferred from physical interaction	24407287	GOA
enables protease binding	IPI IPI: Inferred from physical interaction	24407287	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	11498534	GOA
enables protein dimerization activity	IDA IDA: Inferred from direct assay	24531343	GOA
enables protein homodimerization activity	IDA IDA: Inferred from direct assay	15030775	GOA
enables tropomyosin binding	IPI IPI: Inferred from physical interaction	24407287	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in NLRP3 inflammasome complex assembly	IDA IDA: Inferred from direct assay	27432880	GOA
involved in activation of innate immune response	IDA IDA: Inferred from direct assay	21575908	GOA
involved in cellular response to interleukin-1	IDA IDA: Inferred from direct assay	12486103	GOA
involved in cellular response to lipopolysaccharide	IDA IDA: Inferred from direct assay	12486103	GOA
involved in cellular response to tumor necrosis factor	IDA IDA: Inferred from direct assay	12486103	GOA
involved in defense response to Gram-negative bacterium	IMP IMP: Inferred from mutant phenotype	16982856	GOA
involved in defense response to virus	IDA IDA: Inferred from direct assay	21575908	GOA
involved in intrinsic apoptotic signaling pathway by p53 class mediator	IMP IMP: Inferred from mutant phenotype	14730312	GOA
involved in intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator	IMP IMP: Inferred from mutant phenotype	14730312	GOA
involved in myeloid dendritic cell activation	IMP IMP: Inferred from mutant phenotype	22732093	GOA
involved in negative regulation of NF-kappaB transcription factor activity	IMP IMP: Inferred from mutant phenotype	16585594	GOA
involved in negative regulation of canonical NF-kappaB signal transduction	IDA IDA: Inferred from direct assay	12486103	GOA
involved in negative regulation of cytokine production involved in inflammatory response	IMP IMP: Inferred from mutant phenotype	24531343	GOA
involved in negative regulation of interferon-beta production	IMP IMP: Inferred from mutant phenotype	19158675	GOA
involved in negative regulation of protein serine/threonine kinase activity	IDA IDA: Inferred from direct assay	12486103	GOA
involved in positive regulation of DNA-binding transcription factor activity	IDA IDA: Inferred from direct assay	19494289	GOA
involved in positive regulation of ERK1 and ERK2 cascade	IMP IMP: Inferred from mutant phenotype	21487011	GOA
involved in positive regulation of JNK cascade	IMP IMP: Inferred from mutant phenotype	21487011	GOA
involved in positive regulation of NF-kappaB transcription factor activity	IDA IDA: Inferred from direct assay	12646168	GOA
involved in positive regulation of T cell activation	IMP IMP: Inferred from mutant phenotype	22732093	GOA
involved in positive regulation of adaptive immune response	IMP IMP: Inferred from mutant phenotype	22732093	GOA
involved in positive regulation of apoptotic process	IDA IDA: Inferred from direct assay	12646168	GOA
involved in positive regulation of canonical NF-kappaB signal transduction	IDA IDA: Inferred from direct assay	12656673	GOA
involved in positive regulation of chemokine production	IMP IMP: Inferred from mutant phenotype	21487011	GOA
involved in positive regulation of cysteine-type endopeptidase activity	IDA IDA: Inferred from direct assay	19158676	GOA
involved in positive regulation of cysteine-type endopeptidase activity involved in apoptotic process	IMP IMP: Inferred from mutant phenotype	22267217	GOA
involved in positive regulation of extrinsic apoptotic signaling pathway	IDA IDA: Inferred from direct assay	16964285	GOA
involved in positive regulation of inflammatory response	IDA IDA: Inferred from direct assay	12191486	GOA
involved in positive regulation of interleukin-1 beta production	IDA IDA: Inferred from direct assay	7642516	GOA
involved in positive regulation of interleukin-1 beta production	IMP IMP: Inferred from mutant phenotype	22267217	GOA
involved in positive regulation of interleukin-10 production	IMP IMP: Inferred from mutant phenotype	16982856	GOA
involved in positive regulation of interleukin-6 production	IMP IMP: Inferred from mutant phenotype	16982856	GOA
involved in positive regulation of interleukin-8 production	IMP IMP: Inferred from mutant phenotype	16982856	GOA
involved in positive regulation of non-canonical NF-kappaB signal transduction	IPI IPI: Inferred from physical interaction	15817483	GOA
involved in positive regulation of release of cytochrome c from mitochondria	IDA IDA: Inferred from direct assay	14730312	GOA
involved in positive regulation of tumor necrosis factor production	IMP IMP: Inferred from mutant phenotype	16982856	GOA
involved in protein homooligomerization	IDA IDA: Inferred from direct assay	24531343	GOA
involved in regulation of intrinsic apoptotic signaling pathway	IDA IDA: Inferred from direct assay	14730312	GOA
involved in regulation of tumor necrosis factor-mediated signaling pathway	IMP IMP: Inferred from mutant phenotype	14730312	GOA
involved in tumor necrosis factor-mediated signaling pathway	IDA IDA: Inferred from direct assay	12656673	GOA

Cellular Component GO Annotation	Evidence	References	Source
part of AIM2 inflammasome complex	IDA IDA: Inferred from direct assay	19158676	GOA
part of AIM2 inflammasome complex	IPI IPI: Inferred from physical interaction	24630722	GOA
located in Golgi membrane	IDA IDA: Inferred from direct assay	23229815	GOA
part of IkappaB kinase complex	IDA IDA: Inferred from direct assay	12486103	GOA
part of NLRP1 inflammasome complex	IDA IDA: Inferred from direct assay	12191486	GOA
part of NLRP1 inflammasome complex	IPI IPI: Inferred from physical interaction	17349957	GOA
part of NLRP3 inflammasome complex	IDA IDA: Inferred from direct assay	15030775	GOA
part of NLRP3 inflammasome complex	IPI IPI: Inferred from physical interaction	29255148	GOA
part of NLRP6 inflammasome complex	IDA IDA: Inferred from direct assay	30674671	GOA
part of canonical inflammasome complex	IPI IPI: Inferred from physical interaction	16037825	GOA
located in cytoplasm	IDA IDA: Inferred from direct assay	14730312	GOA
located in mitochondrion	IDA IDA: Inferred from direct assay	14730312	GOA
part of protein-containing complex	IDA IDA: Inferred from direct assay	22267217	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

PYCARD Protein Structure

PYRIN

CARD

0
100
195 a.a.

Protein Preferred Names	Protein Names
apoptosis-associated speck-like protein containing a CARD	caspase recruitment domain-containing protein 5

PYCARD Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	PYCARD	Q9ULZ3	RIPK1	Homo sapiens	Q13546	Anti Tag CoIP	25402006
Intra	PYCARD	Q9ULZ3	BAX	Homo sapiens	Q07812	Confocal	14730312
Intra	PYCARD	Q9ULZ3	CASP1	Homo sapiens	P29466	Anti Bait CoIP	31421660
Intra	PYCARD	Q9ULZ3	CASP1	Homo sapiens	P29466	Anti Bait CoIP	34589402
Intra	PYCARD	Q9ULZ3	CASP1	Homo sapiens	P29466	Anti Bait CoIP	24630722
Intra	PYCARD	Q9ULZ3	CASP1	Homo sapiens	P29466	Anti Bait CoIP	25605870
Intra	PYCARD	Q9ULZ3	AIM2	Homo sapiens	O14862	Pull Down	19158676
Intra	PYCARD	Q9ULZ3	AIM2	Homo sapiens	O14862	EM	24630722
Intra	PYCARD	Q9ULZ3	AIM2	Homo sapiens	O14862	FPS	24630722
Intra	PYCARD	Q9ULZ3	NLRP3	Homo sapiens	Q96P20	EM	24630722
Intra	PYCARD	Q9ULZ3	NLRP3	Homo sapiens	Q96P20	Anti Bait CoIP	31421660
Intra	PYCARD	Q9ULZ3	NLRP3	Homo sapiens	Q96P20	Y2H	11786556
Intra	PYCARD	Q9ULZ3	NLRP3	Homo sapiens	Q96P20	Confocal	34341353
Intra	PYCARD	Q9ULZ3	NLRP3	Homo sapiens	Q96P20	Anti Tag CoIP	34341353
Intra	PYCARD	Q9ULZ3	NLRP3	Homo sapiens	Q96P20	IF	11786556
Intra	PYCARD	Q9ULZ3	NLRP3	Homo sapiens	Q96P20	Anti Tag CoIP	19158675
Intra	PYCARD	Q9ULZ3	PYDC2	Homo sapiens	Q56P42	Pull Down	17178784
Intra	PYCARD	Q9ULZ3	PYDC2	Homo sapiens	Q56P42	Anti Tag CoIP	17178784
Intra	PYCARD	Q9ULZ3	MEFV	Homo sapiens	O15553	Pull Down	25006247
Intra	PYCARD	Q9ULZ3	MEFV	Homo sapiens	O15553	CoIP	11498534
Intra	PYCARD	Q9ULZ3	MEFV	Homo sapiens	O15553	Y2H	11498534
Intra	PYCARD	Q9ULZ3	SYK	Homo sapiens	P43405	Protein Kinase Assay	25605870
Intra	PYCARD	Q9ULZ3	SYK	Homo sapiens	P43405	Anti Bait CoIP	25605870
Intra	PYCARD	Q9ULZ3	SYK	Homo sapiens	P43405	Anti Tag CoIP	25605870
Intra	PYCARD	Q9ULZ3	EXOC5	Homo sapiens	O00471	Y2H Prey Pooling	25416956
Intra	PYCARD	Q9ULZ3	EXOC5	Homo sapiens	O00471	Y2H Array	25416956

Cross: Cross-species interaction Intra: Intraspecies interaction

PYCARD Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P80522	TMS1/ASC Antibody (YA033)	WB, ICC/IF, FC	Human
HY-P80548	TMS1/ASC Antibody	WB, IHC-F, IHC-P, ICC/IF, ELISA	Human, Mouse, Rat
HY-P84876	TMS1/ASC Antibody (YA4573)	IHC-P, ICC/IF, FC, ELISA	Human
HY-P84876A	TMS1/ASC Antibody (YA4573)(PBS only)	IHC-P, ICC/IF, FC, ELISA	Human
HY-P86234	TMS1/ASC Antibody (YA5926)	WB, ICC/IF, IP, ELISA	Mouse, Rat

Related Diseases

Diseases	Alias
Muckle-Wells Syndrome	MWS Urticaria-Deafness-Amyloidosis Syndrome Uda Syndrome Neutrophilic Urticaria Urticaria, Deafness And Amyloidosis Cryopyrin-Associated Periodic Syndrome 2 Caps2 Muckle Wells Syndrome Urticaria-Deafness-Amyloidosis Cryopyrin-Associated Periodic Syndromes
Cinca Syndrome	CINCA Nomid Cryopyrin-Associated Periodic Syndrome 3 Chronic Neurologic Cutaneous And Articular Syndrome Multisystem Inflammatory Disease, Neonatal-Onset Caps3 Chronic Infantile Neurological Cutaneous Articular Syndrome Infantile-Onset Multisystem Inflammatory Disease Iomid Syndrome Neonatal-Onset Multisystem Inflammatory Disease Nomid Syndrome Prieur-Griscelli Syndrome Neonatal Onset Multisystem Inflammatory Disease Chronic Infantile Neurological, Cutaneous And Articular Syndrome Iomid Infantile Onset Multisystem Inflammatory Disease Prieur Griscelli Syndrome Chronic Infantile Neurological Cutaneous And Articular Syndrome Chronic, Infantile, Neurological, Cutaneous, Articular Syndrome Chronic Infantile Neurologic Cutaneous And Articular Syndrome Chronic Infantile Neurological, Cutaneous, And Articular Syndrome Cryopyrin-Associated Periodic Syndromes
Familial Mediterranean Fever	Periodic Fever Syndrome FMF Benign Paroxysmal Peritonitis Periodic Disease Recurrent Polyserositis Familial Paroxysmal Polyserositis Periodic Fever Familial Mediterranean Fever, Autosomal Recessive Familial Mediterranean Fever, Ar Polyserositis, Recurrent Polyserositis, Familial Paroxysmal Periodic Peritonitis Mef Reimann Periodic Disease Siegal-Cattan-Mamou Disease Wolff Periodic Disease Benign Recurrent Polyserositis Mediterranean Fever, Familial ARFMF Autosomal Recessive Familial Mediterranean Fever Fever, Mediterranean, Familial, Autosomal Recessive Hereditary Autoinflammatory Diseases Fmf - [Familial Mediterranean Fever] Periodic Polyserositis Periodic Familial Polyserositis Periodic Familial Peritonitis Paroxysmal Polyserositis Hereditary Amyloid Nephropathy Familial Recurrent Polyserositis Familial Non-Neuropathic Amyloidosis Armenian Disease Riemann Periodic Disease Siegal Cattan Mamou Disease
Osteomyelitis
Gout	Gouty Arthritis Articular Gout Gouty Arthropathy Arthritis, Gouty Arthritis Gouty Idiopathic Gout Idiopathic Gout, Unspecified Site Gouty Bursitis Uratic Arthritis Gout Nos Gouty Gouty Diathesis
Familial Cold Autoinflammatory Syndrome	Familial Cold Urticaria Fcas Familial Polymorphous Cold Eruption Fcu Cold Hypersensitivity
Melanoma	Malignant Melanoma Cutaneous Melanoma Naevocarcinoma Malignant Melanomas
Colorectal Cancer	Colon Cancer Colorectal Carcinoma Colon Carcinoma Colorectal Cancer, Susceptibility To Carcinoma Of Colon CRC Colorectal Cancer With Chromosomal Instability, Somatic Colon Cancer, Somatic Colon Cancer, Susceptibility To Colonic Neoplasms Colorectal Neoplasms Colorectal Cancer, Somatic Colon Cancer, Advanced, Somatic Colonic Carcinoma Colorectal Carcinomas Colon Cancers Colorectal Cancers Cancer, Colorectal, Somatic Cancer, Colon Cancer, Colorectal, Susceptibility To Colorectal Neoplasm Colonic Neoplasm Malignant Tumor Of Colon

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (3)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-RS11485	PYCARD Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS19524	Pycard Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS26019	Pycard Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Mus musculus	PYCARD	MGD	MGI:1931465
Macaca mulatta	PYCARD	VGNC	VGNC:76489
Felis catus	PYCARD	VGNC	VGNC:80676
Rattus norvegicus	PYCARD	RGD	RGD:628637
Others	PYCARD	NCBI