NMB-1

NMB-1 (2-5 µM; 15 min) binds specifically to rat DRG membrane proteins but not to cerebellar proteins^[1].
NMB-1 (5 µM; 3.5 min) is analyzed by pull-down combined with LC-MS/MS, and annexin A6 is the most abundant candidate among the binding proteins shared by DRG neurons and ND-C cells, with a cumulative peptide match of 36^[1].
NMB-1 (2 µM; overnight) binds to WT mouse DRG tissue sections, whereas binding to Anxa6 KO DRG tissue is significantly reduced, with residual binding to other membrane proteins^[1].
NMB-1 (0.15-15 μM) selectively blocks slowly adapting mechanically activated currents in cultured rat DRG neurons, with an apparent IC₅₀ of 1.0 μM^[2].
NMB-1 (2 μM; 2 min) selectively binds peripherin-positive small-diameter cultured adult rat DRG neurons^[2].
NMB-1 (5 μM) inhibits mechanotransduction-dependent rapid FM1-43 loading in rat cochlear inner and outer hair cells^[2].
NMB-1 (2 μM) does not inhibit mustard oil-induced TRPA1 currents in transfected CHO-K1 cells^[2].
NMB-1 (0.1-10 μM; 600 ms) inhibits TTN3-mediated slowly adapting MA currents in HEK293T cells with an IC₅₀ of 0.92 μM^[3].
NMB-1 (10 μM; 600 ms) does not inhibit Piezo1- or Piezo2-mediated MA currents in HEK293T cells, supporting selectivity for TTN3^[3].
NMB-1 (10 μM; 600 ms) inhibition of TTN3 depends on the negative charge of Glu126^[3].