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  4. DMANI

DMANI is an intramolecular charge transfer (ICT)-based mitochondria-targeted ratiometric fluorescent probe. DMANI can monitor mitochondrial peroxynitrite (ONOO−) in living cells.

For research use only. We do not sell to patients.

DMANI

DMANI Chemical Structure

CAS No. : 2978635-30-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

DMANI Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

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Description

DMANI is an intramolecular charge transfer (ICT)-based mitochondria-targeted ratiometric fluorescent probe. DMANI can monitor mitochondrial peroxynitrite (ONOO−) in living cells[1].

In Vitro

In PBS buffer (10 mM, pH = 7.4), the DMANI (5 μM) displays absorption bands at around 525 and 346 nm, but the addition of 30 μM ONOO− significantly decreased the absorption, together with the appearance of a new peak at about 360 nm. In the emission spectra, a remarkably decreased fluorescence was observed at 692 nm and a new emission band at 444 nm appeared and increased rapidly in the case of DMANI with ONOO−[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DMANI Related Antibodies
Molecular Weight

482.40

Formula

C25H27IN2
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

CN(C)C1=CC=C2C(C=CC(/C=C/C(C3(C)C)=[N+](C)C4=C3C=CC=C4)=C2)=C1.[I-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (207.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0730 mL 10.3648 mL 20.7297 mL
5 mM 0.4146 mL 2.0730 mL 4.1459 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0730 mL 10.3648 mL 20.7297 mL 51.8242 mL
5 mM 0.4146 mL 2.0730 mL 4.1459 mL 10.3648 mL
10 mM 0.2073 mL 1.0365 mL 2.0730 mL 5.1824 mL
15 mM 0.1382 mL 0.6910 mL 1.3820 mL 3.4549 mL
20 mM 0.1036 mL 0.5182 mL 1.0365 mL 2.5912 mL
25 mM 0.0829 mL 0.4146 mL 0.8292 mL 2.0730 mL
30 mM 0.0691 mL 0.3455 mL 0.6910 mL 1.7275 mL
40 mM 0.0518 mL 0.2591 mL 0.5182 mL 1.2956 mL
50 mM 0.0415 mL 0.2073 mL 0.4146 mL 1.0365 mL
60 mM 0.0345 mL 0.1727 mL 0.3455 mL 0.8637 mL
80 mM 0.0259 mL 0.1296 mL 0.2591 mL 0.6478 mL
100 mM 0.0207 mL 0.1036 mL 0.2073 mL 0.5182 mL
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DMANI Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DMANI2978635-30-0Fluorescent DyeInhibitorinhibitorinhibit

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