DMT-dC(bz) Phosphoramidite
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DMT-dC (bz) Phosphoramidite is a protected deoxycytidine phosphoramidite monomer, as well as a building block for mechanochemical DNA synthesis. DMT-dC (bz) Phosphoramidite serves as a reagent for oligonucleotide synthesis.
For research use only. We do not sell to patients.
- Purity: 99.44%
- CAS No.: 102212-98-6
- Formula: C46H52N5O8P
- Molecular Weight:833.91
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
More
Biological Activity
DMT-dC(bz) Phosphoramidite (2 equivalents; 30 min at 30 Hz) undergoes mechanochemical coupling with 3'-O-tert-butyldimethylsilyl thymidine to generate the target phosphite triester intermediate in 80% yield[1].
DMT-dC(bz) Phosphoramidite (2 equivalents; 30 min at 30 Hz coupling, 20 min at 30 Hz oxidation, 10 min at 30 Hz detritylation) undergoes one-pot mechanochemical coupling, oxidation, and detritylation with 3'-O-tert-butyldimethylsilyl thymidine to generate the target DNA dimer in 32% yield over three steps[1].
DMT-dC(bz) Phosphoramidite (2 equivalents; 30 min at 30 Hz coupling, 20 min at 30 Hz sulfurization) undergoes mechanochemical coupling with 3'-O-tert-butyldimethylsilyl thymidine followed by in situ sulfurization to generate the target DNA phosphorothioate dimer in 57% yield over two steps[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 102212-98-6
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Appearance Solid
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Molecular Weight 833.91
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Formula C46H52N5O8P
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Color White to off-white
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SMILES
COC(C=C1)=CC=C1C(C2=CC=CC=C2)(C3=CC=C(OC)C=C3)OC[C@@H]4[C@@H](OP(N(C(C)C)C(C)C)OCCC#N)C[C@H](N5C(N=C(NC(C6=CC=CC=C6)=O)C=C5)=O)O4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 100 mg/mL (119.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (3.00 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1992 mL | 5.9959 mL | 11.9917 mL | 29.9793 mL |
| 5 mM | 0.2398 mL | 1.1992 mL | 2.3983 mL | 5.9959 mL | |
| 10 mM | 0.1199 mL | 0.5996 mL | 1.1992 mL | 2.9979 mL | |
| 15 mM | 0.0799 mL | 0.3997 mL | 0.7994 mL | 1.9986 mL | |
| 20 mM | 0.0600 mL | 0.2998 mL | 0.5996 mL | 1.4990 mL | |
| 25 mM | 0.0480 mL | 0.2398 mL | 0.4797 mL | 1.1992 mL | |
| 30 mM | 0.0400 mL | 0.1999 mL | 0.3997 mL | 0.9993 mL | |
| 40 mM | 0.0300 mL | 0.1499 mL | 0.2998 mL | 0.7495 mL | |
| 50 mM | 0.0240 mL | 0.1199 mL | 0.2398 mL | 0.5996 mL | |
| 60 mM | 0.0200 mL | 0.0999 mL | 0.1999 mL | 0.4997 mL | |
| 80 mM | 0.0150 mL | 0.0749 mL | 0.1499 mL | 0.3747 mL | |
| 100 mM | 0.0120 mL | 0.0600 mL | 0.1199 mL | 0.2998 mL |
DMT-dC(bz) Phosphoramidite Related Classifications
HY-W008849 Related Classifications
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- DMT-dC(bz) Phosphoramidite
- 102212-98-6
- Phosphoramidites
- DNA/RNA Synthesis
- Nucleoside Antimetabolite/Analog
- oligonucleotide synthesis
- DNA phosphorothioate dimer
- DNA dimer
- mechanochemical DNA synthesis
- 3'-O-tert-butyldimethylsilyl thymidine
- phosphite triester intermediate
- deoxycytidine phosphoramidite monomer
- Inhibitor
- inhibitor
- inhibit