DPP-8/9 probe-1
DPP-8/9 probe-1 (compound 20) is a fluorescent probe targeting Dipeptidyl Peptidase DPP8/9, which can be selectively labeled and visualized in vitro by fluorescence microscopy Active DPP8/9. DPP-8/9 probe-1 contains a nitrobenzoxadiazole (NBD) tag and has high affinity and selectivity for DPP8/9 over related S9 family members (IC50 of 210 nM and 15 nM, respectively).
For research use only. We do not sell to patients.
- CAS No.: 3020859-80-4
- Formula: C31H36FN7O7
- Molecular Weight:637.66
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
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DPP-8 210 nM (IC50, [1]) |
DPP-9 15 nM (IC50, [1]) |
DPP-4 .44 μM (IC50, [1]) |
In Vitro
DPP-8/9 probe-1 (compound 20) (10 μM; 30 min) significantly stains and visualizes DPP-8/9 in THP cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:THP-1 monocytes
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Concentration:0.5 μM, 1 μM, 2 μM, 10 μM
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Incubation Time:30 min
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Result:Lower concentrations (0.5 μM, 1 μM and 2 μM) can not be sufficient for in-cell staining
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Chemical Information
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CAS No. 3020859-80-4
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Molecular Weight 637.66
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Formula C31H36FN7O7
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SMILES
O=C(CN[C@@]1(C2)C[C@H]3C[C@@](C[C@@H]2C3)(NC(OCCOCCNC4=CC=C([N+]([O-])=O)C5=NON=C54)=O)C1)N(C6)CC7=C6C=CC(F)=C7
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DPP-8/9 probe-1 Related Classifications
HY-D2327 Related Classifications
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