EDTA-AM
Based on 2 publication(s) in Google Scholar
EDTA-AM (ethylenediaminetetraacetic acid, acetoxymethyl ester) is the membrane-permeant form of the metal chelator EDTA (HY-Y0682). Live cells passively load EDTA-AM by incubating with EDTA-AM. Once internalized, cytoplasmic esterase decomposes AM esters, releasing the active ligand EDTA, which isolates metal ions within the cell. EDTA-AM induces an arrest of mitotic progression and chromosome decondensation.
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- Pureté: 98.0%
- CAS No.: 162303-59-5
- Formule: C22H32N2O16
- Masse moléculaire:580.49
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Stockage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) EDTA-AM
More
Activité biologique
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
EDTA-AM treatment of mitotic cells：
1. Mitotic cells were collected and seeded onto a 12-well culture plate with poly lysine-coated coverslips and DMEM supplemented with 10% FBS.
2. EDTA-AM was dissolved in 100 mM DMSO, EDTA-AM was added to the culture medium at a final concentration of 20 mM with 0.02% Pluronic F-127 (Invitrogen), and the cells were further incubated at 37C for 100 min.
3.After incubation, the numbers of mitotic cells and interphase cells were counted. The cells on the coverslips were fixed, stained with DAPI, and mounted with para-phenylene diamine (PPDI) solution (20 mM HEPES, pH 7.4, 1 mM MgCl2, 100 mM KCl, 78% glycerol, 1 mg/mL PPDI).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 162303-59-5
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Appearance Liquid (Density: 1.337±0.06 g/cm3)
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Masse moléculaire 580.49
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Formule C22H32N2O16
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Color Colorless to light yellow
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SMILES
O=C(C)OCOC(CN(CCN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=O
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Synonyms
EDTA acetoxymethyl ester; Ethylenediaminetetraacetic acid acetoxymethyl ester
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (2)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
Inhibition of Calcium-Dependent Lipid Droplets Relocation of ACSL4-PKCβ-ALOX15 Complex Alleviates Ferroptosis and Acute Pancreatitis. [Abstract]2026 Apr;13(19):e15768. PMID: 41589658 -
iScience
2025 Jul 7;28(8):113073. PMID: 40727937
Solvant et solubilité
DMSO : 100 mg/mL (172.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Watkins CS, et.al. Effects on K+ currents in rat cerebellar granule neurones of a membrane-permeable analogue of the calcium chelator BAPTA. Br J Pharmacol. 1996 Aug;118(7):1772-8. [Content Brief]
[2]. Maeshima K, et.al. A Transient Rise in Free Mg2+Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation. Curr Biol. 2018 Feb 5;28(3):444-451.e6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7227 mL | 8.6134 mL | 17.2268 mL | 43.0671 mL |
| 5 mM | 0.3445 mL | 1.7227 mL | 3.4454 mL | 8.6134 mL | |
| 10 mM | 0.1723 mL | 0.8613 mL | 1.7227 mL | 4.3067 mL | |
| 15 mM | 0.1148 mL | 0.5742 mL | 1.1485 mL | 2.8711 mL | |
| 20 mM | 0.0861 mL | 0.4307 mL | 0.8613 mL | 2.1534 mL | |
| 25 mM | 0.0689 mL | 0.3445 mL | 0.6891 mL | 1.7227 mL | |
| 30 mM | 0.0574 mL | 0.2871 mL | 0.5742 mL | 1.4356 mL | |
| 40 mM | 0.0431 mL | 0.2153 mL | 0.4307 mL | 1.0767 mL | |
| 50 mM | 0.0345 mL | 0.1723 mL | 0.3445 mL | 0.8613 mL | |
| 60 mM | 0.0287 mL | 0.1436 mL | 0.2871 mL | 0.7178 mL | |
| 80 mM | 0.0215 mL | 0.1077 mL | 0.2153 mL | 0.5383 mL | |
| 100 mM | 0.0172 mL | 0.0861 mL | 0.1723 mL | 0.4307 mL |
EDTA-AM Related Classifications
HY-D1746 Related Classifications
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