ER-Laurdan 

ER-Laurdan is a derivative of the membrane-permeable fluorescent probe Laurdan (HY-D0080) that targets the endoplasmic reticulum (ER). As a membrane fluidity reporter, ER-Laurdan specifically localizes to the ER luminal membrane, and exhibits a prominent solvatochromic response to fluidity changes caused by membrane packing and exogenous saturated fatty acid stress. ER-Laurdan shows no overlapping localization with mitochondria, enables quantitative analysis of ER membrane fluidity via generalized polarization ratio measurement, and allows automatic signal masking with the help of ER markers. With high specificity and quantitative capability, ER-Laurdan serves as an important tool for investigating metabolic disorders and associated changes in the physical properties of cell membranes^[1][2].

ER-Laurdan (5 μM; 20 min) localizes specifically to the endoplasmic reticulum (ER) in HeLa cells, with high colocalization with the ER marker ER-Tracker Red^[1].
ER-Laurdan (5 μM; 20 min) detects reduced ER membrane fluidity (increased GP) in HeLa cells treated with 200 μM PA (24-48 h), while lower PA concentrations do not induce significant changes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1074.22

C₅₄H₈₀F₅N₃O₁₃

3091879-77-2

Solid

White to light yellow

O=C(C1=C(F)C(F)=C(F)C(F)=C1F)NCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCNC(CN(C)C2=CC=C3C(C=CC(C(CCCCCCCCCCC)=O)=C3)=C2)=O

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Wong AM, et al. Organelle-Targeted Laurdans Measure Heterogeneity in Subcellular Membranes and Their Responses to Saturated Lipid Stress. ACS Chem Biol. 2024;19(8):1773-1785.  [Content Brief]

  [2]. Tsai Y T, et al. 1-Deoxysphingolipids dysregulate membrane properties and cargo trafficking in the early secretory pathway[J]. Cell chemical biology, 2026, 33(1): 45-58. e8.