UP12
Based on 1 Customer Validation
UP12 is an ATPase activity activator that activates the ATPase activity of valosin-containing protein (VCP), with an EC50 of 1.24 μM. UP12 is applicable to research related to multisystem proteinopathies.
For research use only. We do not sell to patients.
- Purity: 97.69%
- CAS No.: 443353-87-5
- Formula: C23H16ClN3O3S
- Molecular Weight:449.91
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
UP12 (0.240-5.00 μM; 1 h) increases the ATPase activity of recombinant wild-type human VCP by 39%; its potency is enhanced in the presence of UFD1/NPLOC4 cofactors, with the underlying mechanism involving an increase in catalytic turnover efficiency and a slight improvement in ATP affinity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 443353-87-5
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Appearance Solid
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Molecular Weight 449.91
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Formula C23H16ClN3O3S
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Color Off-white to light yellow
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SMILES
O=C(C1=CC=CC=C1)NN2C(SCC(C3=CC=C(C=C3)Cl)=O)=NC4=C(C2=O)C=CC=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (222.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.56 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2227 mL | 11.1133 mL | 22.2267 mL | 55.5667 mL |
| 5 mM | 0.4445 mL | 2.2227 mL | 4.4453 mL | 11.1133 mL | |
| 10 mM | 0.2223 mL | 1.1113 mL | 2.2227 mL | 5.5567 mL | |
| 15 mM | 0.1482 mL | 0.7409 mL | 1.4818 mL | 3.7044 mL | |
| 20 mM | 0.1111 mL | 0.5557 mL | 1.1113 mL | 2.7783 mL | |
| 25 mM | 0.0889 mL | 0.4445 mL | 0.8891 mL | 2.2227 mL | |
| 30 mM | 0.0741 mL | 0.3704 mL | 0.7409 mL | 1.8522 mL | |
| 40 mM | 0.0556 mL | 0.2778 mL | 0.5557 mL | 1.3892 mL | |
| 50 mM | 0.0445 mL | 0.2223 mL | 0.4445 mL | 1.1113 mL | |
| 60 mM | 0.0370 mL | 0.1852 mL | 0.3704 mL | 0.9261 mL | |
| 80 mM | 0.0278 mL | 0.1389 mL | 0.2778 mL | 0.6946 mL | |
| 100 mM | 0.0222 mL | 0.1111 mL | 0.2223 mL | 0.5557 mL |
UP12 Related Classifications
HY-168325 Related Classifications
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