Phosphoramide mustard
Based on 8 publication(s) in Google Scholar
Phosphoramide mustard is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard induces DNA damage.
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- No. CAS: 10159-53-2
- Fòrmula: C4H11Cl2N2O2P
- Peso molecular:221.02
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) Phosphoramide mustard
More- Sci Adv. 2026 Apr 3;12(14):eaec8684. [Abstract]
- Stem Cell Res Ther. 2024 Apr 5;15(1):97. [Abstract]
- Int J Nanomedicine. 2025 Aug 22:20:10195-10212. [Abstract]
- J Funct Foods. 2025 Jun.
- Sci Rep. 2024 Aug 16;14(1):19008. [Abstract]
- Climacteric. 2025 Jan 10:1-12. [Abstract]
- Tufts University. 2025.
- SSRN. 2024 Mar 21.
Customer Validation & Images
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Cell Proliferation/Viability Assay
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Bio/Physico-chemical Assay
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Cell Imaging/Staining
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WB
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Cell Imaging/Staining
Actividad biológica
DNA Alkylator[1]
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Cell Line
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Type | Value | Description | References |
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| V79 | IC50 |
77 μM
Compound: 27
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In vitro cytotoxicity against V-79 chinese hamster lung fibroblasts (3 hr drug exposure time) by clonogenic assay
In vitro cytotoxicity against V-79 chinese hamster lung fibroblasts (3 hr drug exposure time) by clonogenic assay
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[PMID: 15239662] |
Phosphoramide mustard causes cytotoxicity through forming cross-linked DNA adducts which inhibit DNA strand separation during replication[1].
Phosphoramide mustard (3-6 μM; 48 hours) reduces cell viability in rat spontaneously immortalized granulosa cells (SIGCs)[1].
Phosphoramide mustard (3-6 μM; 24-48 hours) induces DNA adduct formation and ovarian DNA damage[1].
Phosphoramide mustard (3-6 μM; 24-48 hours) increases DNA damage responses (DDR) gene mRNA expression levels and DDR proteins[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:SIGCs
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Concentration:0.5 μM, 1 μM, 3 μM, 6 μM
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Incubation Time:48 hours
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Result:Reduced cell viability at concentrations of 3 μM and higher.
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Cell Line:SIGCs
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Concentration:3 μM, 6 μM
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Incubation Time:24 hours, 48 hours
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Result:Increased DDR gene mRNA expression levels.
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Cell Line:SIGCs
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Concentration:3 μM, 6 μM
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Incubation Time:24 hours, 48 hours
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Result:Generally increased DDR proteins.
Phosphoramide mustard exhibits terminal elimination half-lives (rat 15.1 min) following intravenous administration (rat 59.4 mg/kg)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Rat, subcutaneously implanted Walker 256 carcinosarcoma tumor[2]
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Dosage:2.1 mg/kg, 4.8 mg/kg, 10.4 mg/kg, 20.7 mg/kg
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Administration:Intraperitoneal injection, once daily, for 5 consecutive days
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Result:Required to produce 50% inhibition of subcutaneous tumor growth with dose of 12 mg/kg.
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Animal Model:Rats[2]
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Dosage:59.4 mg/kg (Pharmacokinetic Analysis)
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Administration:Intravenous injection
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Result:T1/2 (15.1 min).
Chemical Information
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No. CAS 10159-53-2
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Peso molecular 221.02
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Fòrmula C4H11Cl2N2O2P
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SMILES
O=P(N(CCCl)CCCl)(N)O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (8)
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Journal Impact Factor
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Most Recent
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Sci Adv
Regulation of mitochondrial ROS by C15ORF48 in a basal cell subpopulation contributes to chemotherapy resistance in TNBC. [Abstract]2026 Apr 3;12(14):eaec8684. PMID: 41931605 -
Stem Cell Res Ther
Pyrroloquinoline quinone promotes human mesenchymal stem cell-derived mitochondria to improve premature ovarian insufficiency in mice through the SIRT1/ATM/p53 pathway. [Abstract]2024 Apr 5;15(1):97. PMID: 38581065 -
Int J Nanomedicine
Exosomes Derived From Human Mesenchymal Stem Cells Mitigate Follicular Interstitial Cell Ferroptosis via the miR-26a-5p/PTEN/GPX4 Axis in Rats with Chemotherapy-Induced Premature Ovarian Insufficiency. [Abstract]2025 Aug 22:20:10195-10212. PMID: 40873678 -
Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.
Phosphoramide mustard cyclohexanamine (PM) (3–120 μM; 48 h) significantly inhibited Caco-2 cell viability compared with the control-treated group.
Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.
Phosphoramide mustard cyclohexanamine (PM) (15–120 μM; 48 h) (PM) promoted the increase of MDA content in Caco-2 cells in a concentration-dependent manner.
Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.
Phosphoramide mustard cyclohexanamine (PM) (15–120 μM; 48 h) significantly enhanced the red fluorescence intensity emitted by RhoNox-1 staining, indicating the specific accumulation of Fe2+ in Caco-2 cells.
Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.
Phosphoramide mustard cyclohexanamine (PM) (15–120 μM; 48 h) treatment of Caco-2 cells caused overexpression of NCOA4 protein in a concentration-dependent manner.
Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.
Phosphoramide mustard cyclohexanamine (PM) (30 μM; 48 h) induced autophagy in Caco-2 cells, as evidenced by increased autophagosomes and autophagolysosomes.
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Sci Rep
hUMSCs restore ovarian function in POI mice by regulating GSK3β-mediated mitochondrial dynamic imbalances in theca cells. [Abstract]2024 Aug 16;14(1):19008. PMID: 39152165 -
Climacteric
Intravenous administration of mitochondria improves ovarian function by anti-apoptosis in the premature ovarian insufficiency model. [Abstract]2025 Jan 10:1-12. PMID: 39791362 -
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Pureza y Documentación
Referencias
[1]. Shanthi Ganesan, et al. Phosphoramide mustard exposure induces DNA adduct formation and the DNA damage repair response in rat ovarian granulosa cells. Toxicol Appl Pharmacol. 2015 Feb 1; 282(3): 252–258. [Content Brief]
[2]. S Genka, et al. Brain and plasma pharmacokinetics and anticancer activities of cyclophosphamide and phosphoramide mustard in the rat. Cancer Chemother Pharmacol. 1990;27(1):1-7. [Content Brief]
Calculators
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