Esculin sesquihydrate
Based on 5 publication(s) in Google Scholar
Esculin sesquihydrate, a fluorescent coumarin glucoside, is an active ingredient of ash bark. Esculin sesquihydrate ameliorates cognitive impairment in experimental diabetic nephropathy (DN), and exerts anti?oxidative stress and anti?inflammatory effects, via the MAPK signaling pathway.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity : 98.80%
- CAS No.: 66778-17-4
- 화학식: C15H16O9.3/2H2O
- 분자량:367.31
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보관:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
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Stigmasterol (Standard)Standard of Stigmasterol
Publications Citing Use of MedChemExpress (MCE) Esculin sesquihydrate
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Customer Validation & Images
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Cell Proliferation/Viability Assay
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IF
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Flow Cytometry
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WB
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IHC
Biological Activity
제품 설명
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 66778-17-4
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Appearance Solid
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분자량 367.31
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화학식 C15H16O9.3/2H2O
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Color White to off-white
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SMILES
O=C1C=CC2=CC(O[C@H]3[C@@H]([C@H]([C@@H]([C@@H](CO)O3)O)O)O)=C(O)C=C2O1.O.[3/2]
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (5)
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Journal Impact Factor
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Most Recent
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Phytomedicine
Aesculin suppresses the NLRP3 inflammasome-mediated pyroptosis via the Akt/GSK3β/NF-κB pathway to mitigate myocardial ischemia/reperfusion injury. [Abstract]2021 Nov:92:153687. PMID: 34482222 -
Int Immunopharmacol
Esculin suppresses the PERK-eIF2α-CHOP pathway by enhancing SIRT1 expression in oxidative stress-induced rat chondrocytes, mitigating osteoarthritis progression in a rat model. [Abstract]2024 May 10:132:112061. PMID: 38608474
Esculin sesquihydrate purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 May 10:132:112061. [Abstract]
The viability of TBHP-treated (60 μM) chondrocytes after Esculin (0, 50, 100, 200, 300, 400, 500, 600 μM) treatment.
Esculin sesquihydrate purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 May 10:132:112061. [Abstract]
Apoptotic chondrocytes were identified through TUNEL fluorescence immunocytochemistry treated with Esculin (300 μM).
Esculin sesquihydrate purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 May 10:132:112061. [Abstract]
Flow cytometry was employed to assess chondrocyte apoptosis, with Annexin V and PI fluorescence labeling for detection treated with Esculin (300 μM).
Esculin sesquihydrate purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 May 10:132:112061. [Abstract]
The protein levels of Bcl2, Bax, and cleaved caspase3 were measured by western blot treated with Esculin (300 μM).
Esculin sesquihydrate purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2024 May 10:132:112061. [Abstract]
IHC staining was performed to assess the expression of cleaved caspase3, CHOP, and SIRT1 treated with Esculin (200, 400 μg/kg, knee joints injection).
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Plant Methods
Regulating bulb dormancy release and flowering in lily through chemical modulation of intercellular communication. [Abstract]2023 Nov 28;19(1):136. PMID: 38012626 -
Comput Biol Chem
Integrated strategy of serum metabolomics, network pharmacology and experimental validation on revealing the bioactive metabolites and mechanism of Lycium ruthenicum Murr. against colorectal carcinoma. [Abstract]2025 Oct 15;120(Pt 2):108721. PMID: 41105991 -
Plant Direct
Evaluation of pathways to the C-glycosyl isoflavone puerarin in roots of kudzu ( Pueraria montana lobata). [Abstract]2022 Sep 2;6(9):e442. PMID: 36091880
용액&용해도
In Vitro:
DMSO : 10 mg/mL (27.22 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (2.72 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
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Alzheimer’s Disease Modeling
Alzheimer’s Disease (AD) is a neurodegenerative disorder characterized by a progressive decline in cognitive functions and loss of specific types of neurons and synapses. Alzheimer's symptoms can be simulated in mice by injecting drugs (such as Aβ) or genetically modified.
순도&문서
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Knox K, et al. The Coumarin Glucoside, Esculin, Reveals Rapid Changes in Phloem-Transport Velocity in Response to Environmental Cues. Plant Physiol. 2018 Oct;178(2):795-807. [Content Brief]
[2]. Song Y, et al. Esculin ameliorates cognitive impairment in experimental diabetic nephropathy and induces anti-oxidative stress and anti-inflammatory effects via the MAPK pathway. Mol Med Rep. 2018 May;17(5):7395-7402. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.7225 mL | 13.6125 mL | 27.2250 mL | 68.0624 mL |
| 5 mM | 0.5445 mL | 2.7225 mL | 5.4450 mL | 13.6125 mL | |
| 10 mM | 0.2722 mL | 1.3612 mL | 2.7225 mL | 6.8062 mL | |
| 15 mM | 0.1815 mL | 0.9075 mL | 1.8150 mL | 4.5375 mL | |
| 20 mM | 0.1361 mL | 0.6806 mL | 1.3612 mL | 3.4031 mL | |
| 25 mM | 0.1089 mL | 0.5445 mL | 1.0890 mL | 2.7225 mL |
Esculin sesquihydrate Related Classifications
HY-W166297 Related Classifications
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