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Cat. No.: HY-D1246

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Ethidium monoazide bromide

Ethidium monoazide bromide is a DNA intercalating fluorescent dye that enters bacteria with damaged membranes. Ethidium monoazide bromide can be covalently linked to DNA by photoactivation. Ethidium monoazide bromide stains only dead cells. Ethidium monoazide (bromide) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

For research use only. We do not sell to patients.

CAS No.: 58880-05-0
Formula: C₂₁H₁₈BrN₅
Molecular Weight:420.31

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (268 KB)

Ethidium monoazide bromide Chemical Structure

CAS No.: 58880-05-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Emission (Em)
- Excitation (Ex)
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Ethidium monoazide bromide is a DNA intercalating fluorescent dye that enters bacteria with damaged membranes. Ethidium monoazide bromide can be covalently linked to DNA by photoactivation. Ethidium monoazide bromide stains only dead cells^[1]. Ethidium monoazide (bromide) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

Emission (Em)

600

Excitation (Ex)

504

Chemical Information

CAS No. 58880-05-0
Molecular Weight 420.31
Formula C₂₁H₁₈BrN₅
SMILES

CC[N+]1=C(C2=CC=CC=C2)C3=CC(N=[N+]=[N-])=CC=C3C4=C1C=C(N)C=C4.[Br-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (268 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Nogva HK, et al. Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5'-nuclease PCR. Biotechniques. 2003 Apr;34(4):804-8, 810, 812-3. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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