Ferroptosis inducer-12
Based on 1 Customer Validation
Ferroptosis inducer-12 is a potent and selective ferroptosis inducer. Ferroptosis inducer-12 exhibits potent antiproliferative activity against HT1080 and OS-RC-2, with IC50 values of 3 nM and 5 nM, respectively. Ferroptosis inducer-12 strongly inhibits GPX4 enzymatic activity, induces intracellular ROS and elevates intracellular Fe2+ levels in OS-RC-2 cells. Ferroptosis inducer-12 significantly inhibits tumor growth in BALB/c nude mice bearing OS-RC-2 xenografts. Ferroptosis inducer-12 can be used for the study of cancer.
For research use only. We do not sell to patients.
- Purity: 96.32%
- Formula: C34H29FeN3O4
- Molecular Weight:599.46
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Ferroptosis inducer-12 (Compound A6) (48 h) exhibits potent antiproliferative activity against HT1080 and OS-RC-2, with IC50 values of 3 nM (HT1080) and 5 nM (OS-RC-2)[1].
Ferroptosis inducer-12 (1 μM, 2 h) strongly inhibits glutathione peroxidase 4 (GPX4) enzymatic activity in OS-RC-2 cells[1].
Ferroptosis inducer-12 (0.5 μM, 4 h) induces intracellular ROS, elevates intracellular Fe2+ levels and promotes lipid peroxidation (LPO) accumulation in OS-RC-2 cells[1].
Ferroptosis inducer-12 (10 nM, 24 h) significantly suppresses migration and reduces invasive capacity of OS-RC-2 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:OS-RC-2 cells (2 × 106 in 100 μL PBS) were subcutaneously implanted into the right limb subcutaneous fat of 4-week-old male BALB/c nude mice (weight: 14-16 g)[1]
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Dosage:25 mg/kg, 50 mg/kg
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Administration:i.p., once every 3 days for 27 days
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Result:Showed tumor growth inhibition rate of 44.59% at 50 mg/kg, and reduced tumor volume and weight.
Caused no obvious histological damage to major organs (heart, liver, spleen, lung, kidney) in BALB/c nude mice.
Chemical Information
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Appearance Solid
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Molecular Weight 599.46
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Formula C34H29FeN3O4
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Color Light yellow to yellow
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SMILES
O=C(C#C)N([C@@H](C(OC)=O)C1)[C@@H](C2=CC=C(C(NCc3cccc3)=O)C=C2)C4=C1C5=CC=CC=C5N4.[][Fe][].c6cccc6
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (166.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.17 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6682 mL | 8.3408 mL | 16.6817 mL | 41.7042 mL |
| 5 mM | 0.3336 mL | 1.6682 mL | 3.3363 mL | 8.3408 mL | |
| 10 mM | 0.1668 mL | 0.8341 mL | 1.6682 mL | 4.1704 mL | |
| 15 mM | 0.1112 mL | 0.5561 mL | 1.1121 mL | 2.7803 mL | |
| 20 mM | 0.0834 mL | 0.4170 mL | 0.8341 mL | 2.0852 mL | |
| 25 mM | 0.0667 mL | 0.3336 mL | 0.6673 mL | 1.6682 mL | |
| 30 mM | 0.0556 mL | 0.2780 mL | 0.5561 mL | 1.3901 mL | |
| 40 mM | 0.0417 mL | 0.2085 mL | 0.4170 mL | 1.0426 mL | |
| 50 mM | 0.0334 mL | 0.1668 mL | 0.3336 mL | 0.8341 mL | |
| 60 mM | 0.0278 mL | 0.1390 mL | 0.2780 mL | 0.6951 mL | |
| 80 mM | 0.0209 mL | 0.1043 mL | 0.2085 mL | 0.5213 mL | |
| 100 mM | 0.0167 mL | 0.0834 mL | 0.1668 mL | 0.4170 mL |
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