2. Apoptosis NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
  3. Ferroptosis Reactive Oxygen Species (ROS)
  4. Ferroptosis inducer-12

Ferroptosis inducer-12 is a potent and selective ferroptosis inducer. Ferroptosis inducer-12 exhibits potent antiproliferative activity against HT1080 and OS-RC-2, with IC50 values of 3 nM and 5 nM, respectively. Ferroptosis inducer-12 strongly inhibits GPX4 enzymatic activity, induces intracellular ROS and elevates intracellular Fe2+ levels in OS-RC-2 cells. Ferroptosis inducer-12 significantly inhibits tumor growth in BALB/c nude mice bearing OS-RC-2 xenografts. Ferroptosis inducer-12 can be used for the study of cancer.

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Ferroptosis inducer-12

Ferroptosis inducer-12 Chemical Structure

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Ferroptosis inducer-12 Related Antibodies

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Description

Ferroptosis inducer-12 is a potent and selective ferroptosis inducer. Ferroptosis inducer-12 exhibits potent antiproliferative activity against HT1080 and OS-RC-2, with IC50 values of 3 nM and 5 nM, respectively. Ferroptosis inducer-12 strongly inhibits GPX4 enzymatic activity, induces intracellular ROS and elevates intracellular Fe2+ levels in OS-RC-2 cells. Ferroptosis inducer-12 significantly inhibits tumor growth in BALB/c nude mice bearing OS-RC-2 xenografts. Ferroptosis inducer-12 can be used for the study of cancer[1].

In Vitro

Ferroptosis inducer-12 (Compound A6) (48 h) exhibits potent antiproliferative activity against HT1080 and OS-RC-2, with IC50 values of 3 nM (HT1080) and 5 nM (OS-RC-2)[1].
Ferroptosis inducer-12 (1 μM, 2 h) strongly inhibits glutathione peroxidase 4 (GPX4) enzymatic activity in OS-RC-2 cells[1].
Ferroptosis inducer-12 (0.5 μM, 4 h) induces intracellular ROS, elevates intracellular Fe2+ levels and promotes lipid peroxidation (LPO) accumulation in OS-RC-2 cells[1].
Ferroptosis inducer-12 (10 nM, 24 h) significantly suppresses migration and reduces invasive capacity of OS-RC-2 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ferroptosis inducer-12 Related Antibodies

Cell Migration Assay [1]

Cell Line: OS-RC-2 cells
Concentration: 10 nM
Incubation Time: 24 h
Result: Suppressed migration of OS-RC-2 cells.

Cell Invasion Assay[1]

Cell Line: OS-RC-2 cells
Concentration: 10 nM
Incubation Time: 24 h
Result: Reduced invasive capacity of OS-RC-2 cells.
In Vivo

Ferroptosis inducer-12 (Compound A6) (25-50 mg/kg, i.p., once every 3 days for 27 days) significantly inhibits tumor growth in BALB/c nude mice bearing OS-RC-2 xenografts[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: OS-RC-2 cells (2 × 106 in 100 μL PBS) were subcutaneously implanted into the right limb subcutaneous fat of 4-week-old male BALB/c nude mice (weight: 14-16 g)[1]
Dosage: 25 mg/kg, 50 mg/kg
Administration: i.p., once every 3 days for 27 days
Result: Showed tumor growth inhibition rate of 44.59% at 50 mg/kg, and reduced tumor volume and weight.
Caused no obvious histological damage to major organs (heart, liver, spleen, lung, kidney) in BALB/c nude mice.
Molecular Weight

599.46

Formula

C34H29FeN3O4
Appearance

Solid

SMILES

O=C(C#C)N([C@@H](C(OC)=O)C1)[C@@H](C2=CC=C(C(NCc3cccc3)=O)C=C2)C4=C1C5=CC=CC=C5N4.[][Fe][].c6cccc6
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (166.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6682 mL 8.3408 mL 16.6817 mL
5 mM 0.3336 mL 1.6682 mL 3.3363 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.17 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 96.32%

COA (259 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6682 mL 8.3408 mL 16.6817 mL 41.7042 mL
5 mM 0.3336 mL 1.6682 mL 3.3363 mL 8.3408 mL
10 mM 0.1668 mL 0.8341 mL 1.6682 mL 4.1704 mL
15 mM 0.1112 mL 0.5561 mL 1.1121 mL 2.7803 mL
20 mM 0.0834 mL 0.4170 mL 0.8341 mL 2.0852 mL
25 mM 0.0667 mL 0.3336 mL 0.6673 mL 1.6682 mL
30 mM 0.0556 mL 0.2780 mL 0.5561 mL 1.3901 mL
40 mM 0.0417 mL 0.2085 mL 0.4170 mL 1.0426 mL
50 mM 0.0334 mL 0.1668 mL 0.3336 mL 0.8341 mL
60 mM 0.0278 mL 0.1390 mL 0.2780 mL 0.6951 mL
80 mM 0.0209 mL 0.1043 mL 0.2085 mL 0.5213 mL
100 mM 0.0167 mL 0.0834 mL 0.1668 mL 0.4170 mL
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Ferroptosis inducer-12 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ferroptosis inducer-12Ferroptosis inducer12Ferroptosis inducer 12FerroptosisReactive Oxygen Species (ROS)CancerferroptosisHT1080OS-RC-2ROSInhibitorinhibitorinhibit

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