country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

Cat. No.: HY-170462

Copy Link Print Information

CSPD substrate (25 mM)

CSPD substrate (25 mM) is a chemiluminescent alkaline phosphatase substrate. CSPD substrate (25 mM) can be cleaved by alkaline phosphatase to generate a chemiluminescent signal for detecting the activity of this enzyme.

For research use only. We do not sell to patients.

CAS No.: 142849-53-4
Formula: C₁₈H₂₀ClNa₂O₇P
Molecular Weight:460.75

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (292 KB)

CSPD substrate (25 mM) Chemical Structure

CAS No.: 142849-53-4

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

CSPD substrate can be used to evaluate bone morphogenetic protein-2 (BMP-2)-induced osteogenic differentiation. In two osteoprogenitor cell lines, W20-17 and C3H10T1/2, the CSPD assay successfully monitors the upregulation of endogenous alkaline phosphatase activity following rhBMP-2 stimulation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 142849-53-4
Molecular Weight 460.75
Formula C₁₈H₂₀ClNa₂O₇P
SMILES

O=P(OC1=CC=CC(C2(OC)OOC23C4CC5CC3CC(C5)(C4)Cl)=C1)(O[Na])O[Na]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (292 KB)
SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Blum JS, et al. An optimized method for the chemiluminescent detection of alkaline phosphatase levels during osteodifferentiation by bone morphogenetic protein 2. J Cell Biochem. 2001;80(4):532-537. [Content Brief]

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

Get Quote In-stock

Other size

Get Quote