Biotin methyl ester
Based on 1 Customer Validation
Biotin methyl ester is a cell-permeable precursor of Biotin (HY-B0511). Biotin derived from Biotin methyl ester can bind Streptavidin (HY-P3152).
For research use only. We do not sell to patients.
- Purity : 99.60%
- CAS No.: 608-16-2
- Formula: C11H18N2O3S
- Molecular Weight:258.34
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Biotin methyl ester (1-40 µM) rapidly and synchronously releases SBP-tagged cargo at concentrations as low as 1 µM in the HeLa RUSH system, and this process is independent of SMVT[2].
Biotin methyl ester (1-400 µM) enhances ER-localized TurboID biotinylation in HeLa cells[2].
Biotin methyl ester enhances TurboID biotinylation in MDCK cells[2].
Biotin methyl ester mediates TurboID biotinylation in HEK293T cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 608-16-2
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Appearance Solid
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Molecular Weight 258.34
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Formula C11H18N2O3S
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Color White to off-white
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SMILES
O=C1N[C@@]([H])([C@]([H])(CS2)N1)[C@@H]2CCCCC(OC)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (193.54 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (19.35 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.8709 mL | 19.3543 mL | 38.7087 mL | 96.7717 mL |
| 5 mM | 0.7742 mL | 3.8709 mL | 7.7417 mL | 19.3543 mL | |
| 10 mM | 0.3871 mL | 1.9354 mL | 3.8709 mL | 9.6772 mL | |
| 15 mM | 0.2581 mL | 1.2903 mL | 2.5806 mL | 6.4514 mL | |
| 20 mM | 0.1935 mL | 0.9677 mL | 1.9354 mL | 4.8386 mL | |
| 25 mM | 0.1548 mL | 0.7742 mL | 1.5483 mL | 3.8709 mL | |
| 30 mM | 0.1290 mL | 0.6451 mL | 1.2903 mL | 3.2257 mL | |
| 40 mM | 0.0968 mL | 0.4839 mL | 0.9677 mL | 2.4193 mL | |
| 50 mM | 0.0774 mL | 0.3871 mL | 0.7742 mL | 1.9354 mL | |
| 60 mM | 0.0645 mL | 0.3226 mL | 0.6451 mL | 1.6129 mL | |
| 80 mM | 0.0484 mL | 0.2419 mL | 0.4839 mL | 1.2096 mL | |
| 100 mM | 0.0387 mL | 0.1935 mL | 0.3871 mL | 0.9677 mL |
Biotin methyl ester Related Classifications
HY-W107202 Related Classifications
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