Tiron
Based on 1 publication(s) in Google Scholar
Tiron is a non-toxic chelator of a variety of metals. Tiron is cell permeable analog of vitamin E and function as hydroxyl radical and superoxide scavenger. Tiron is an orally active antioxidant. Tiron can be used to alleviate acute metal overload in animals.
For research use only. We do not sell to patients.
- Purity: 99.94%
- CAS No.: 149-45-1
- Formula: C6H4Na2O8S2
- Molecular Weight:314.20
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Tiron
More
Customer Validation & Images
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Bio/Physico-chemical Assay
Biological Activity
Tiron (10 mM) protects Chinese hamster V79 cells against H2O2-induced cytotoxicity[1].
Tiron (0-20 mM) protects supercoiled DNA from metal-mediated superoxide-dependent strand breaks[1].
Tiron (50 nM-200 nM, 48 h) inhibits HG-induced neonatal rat cardiomyocytes apoptosis[3].
Tiron (50 nM-200 nM, 48 h) reduces intracellular osteopontin in neonatal rat cardiomyocytes[3].
Tiron (0.2 mM, 2 h) inhibits UVB-induced up-regulation of MMP-1 and MMP-3 in HDFs[4].
Tiron (0.7 mM, 48 h) increases the percentage of PT4 cells in both the S and G2/M phases[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:PT4 cells
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Concentration:0.7 mM
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Incubation Time:48 h
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Result:Increased the percentage of PT4 cells in S and G2/M phases, along with a reduction of cells in the G0/G1 phase.
Tiron (300 mg/kg, i.p., daily for two weeks) alleviated apoptosis of the left ventricular cardiomyocytes in STZ-induced diabetic mice[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c mice challenged with Ovalbumin (OVA) aerosol for 8 weeks[2]
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Dosage:200 mg/kg
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Administration:Oral gavage.
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Result:Inhibited levels of NOx, IL-13 and TGF-β1, and immunoreactivity of NF-κB.
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Animal Model:STZ-induced diabetic mice[3]
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Dosage:300 mg/kg
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Administration:i.p., daily for two weeks.
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Result:Reduced the levels of total oxidized proteins and advanced glycation end products (AGEs)-related proteins.
Inhibited cardiac myocyte apoptosis.
Decreased PKCδ localization on plasma membrane.
Chemical Information
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CAS No. 149-45-1
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Appearance Solid
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Molecular Weight 314.20
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Formula C6H4Na2O8S2
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Color Off-white to light yellow
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SMILES
O=S(C1=C(O)C(O)=CC(S(=O)([O-])=O)=C1)([O-])=O.[Na+].[Na+]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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Redox Rep
Flavonoids from Polypodium hastatum as neuroprotective agents attenuate cerebral ischemia/reperfusion injury in vitro and in vivo via activating Nrf2. [Abstract]2025 Dec;30(1):2440204. PMID: 39702961
Tiron purchased from MedChemExpress. Usage Cited in: Redox Rep. 2025 Dec;30(1):2440204. [Abstract]
Tiron (10 mM) reversed OGD/R induced overproduction of ROS in PC12 cells.
Solvent & Solubility
DMSO : 80 mg/mL (254.61 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.62 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
[1]. Krishna CM, et al. The catecholic metal sequestering agent 1,2-dihydroxybenzene-3,5-disulfonate confers protection against oxidative cell damage. Arch Biochem Biophys. 1992 Apr;294(1):98-106. 2. [Content Brief]
[2]. El-Sherbeeny NA, et al. Tiron ameliorates oxidative stress and inflammation in a murine model of airway remodeling. Int Immunopharmacol. 2016 Oct;39:172-180. [Content Brief]
[3]. Jiang P, et al. Tiron ameliorates high glucose-induced cardiac myocyte apoptosis by PKCδ-dependent inhibition of osteopontin. Clin Exp Pharmacol Physiol. 2017 Jul;44(7):760-770. [Content Brief]
[4]. Lu J, et al. Tiron Inhibits UVB-Induced AP-1 Binding Sites Transcriptional Activation on MMP-1 and MMP-3 Promoters by MAPK Signaling Pathway in Human Dermal Fibroblasts. PLoS One. 2016 Aug 3;11(8):e0159998. [Content Brief]
[5]. Monticone M, et al. NAC, tiron and trolox impair survival of cell cultures containing glioblastoma tumorigenic initiating cells by inhibition of cell cycle progression. PLoS One. 2014 Feb 28;9(2):e90085. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1827 mL | 15.9134 mL | 31.8269 mL | 79.5672 mL |
| 5 mM | 0.6365 mL | 3.1827 mL | 6.3654 mL | 15.9134 mL | |
| 10 mM | 0.3183 mL | 1.5913 mL | 3.1827 mL | 7.9567 mL | |
| 15 mM | 0.2122 mL | 1.0609 mL | 2.1218 mL | 5.3045 mL | |
| 20 mM | 0.1591 mL | 0.7957 mL | 1.5913 mL | 3.9784 mL | |
| 25 mM | 0.1273 mL | 0.6365 mL | 1.2731 mL | 3.1827 mL | |
| 30 mM | 0.1061 mL | 0.5304 mL | 1.0609 mL | 2.6522 mL | |
| 40 mM | 0.0796 mL | 0.3978 mL | 0.7957 mL | 1.9892 mL | |
| 50 mM | 0.0637 mL | 0.3183 mL | 0.6365 mL | 1.5913 mL | |
| 60 mM | 0.0530 mL | 0.2652 mL | 0.5304 mL | 1.3261 mL | |
| 80 mM | 0.0398 mL | 0.1989 mL | 0.3978 mL | 0.9946 mL | |
| 100 mM | 0.0318 mL | 0.1591 mL | 0.3183 mL | 0.7957 mL |
Tiron Related Classifications
HY-D0261 Related Classifications
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