BAY-524
Based on 1 publication(s) in Google Scholar
BAY-524 is a potent Bub1 kinase inhibitor. BAY-524 inhibits the recombinant catalytic domain of human Bub1 with an IC50 of 450 nM. BAY-524 can be used for the research of anti-cancer in combination with other agents.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.36%
- No. CAS: 1445830-39-6
- Fòrmula: C24H24F2N6O3
- Peso molecular:482.48
-
Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
-
Solvente y solubilidad
- Pureza y Documentación
- Referencias
-
Help & FAQs
-
Heterocyclic Compound Library
HY-L138
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Bioactive Compound Library
HY-L001
-
Protein Kinase Compound Library
HY-L196
-
High-Throughput Bioactive Compound Library
HY-L205
-
Metabolism/Protease Compound Library
HY-L012
-
Anti-Cancer Compound Library
HY-L025
Publications Citing Use of MedChemExpress (MCE) BAY-524
More
Actividad biológica
IC50: 450 nM (human Bub1)[1]
BAY-524 inhibits the recombinant catalytic domain of human Bub1 with an IC50 of 450 nM[1].
BAY-524 (7-10 μM or 0-30 μM; 14 h or 1 h) specifically inhibit Bub1 kinase[1].
BAY-524 (7 μM , 12 h or 7 μM, 12 μM, 3 h) affects Sgo1 and Sgo2 localization and chromatid cohesion in cells[1].
BAY-524 (7 μM, 10 μM) affects localization and activity of the CPC[1].
BAY-524 (7 μM, 2 h) exerts additive effect on centromere association of CPC[1].
BAY-524 (7 μM, 48 h) marginally affects SAC signaling[1].
BAY-524 (7 μM, 10 μM) sensitizes cells to low doses of paclitaxel[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:hTERT-RPE1 (RPE1) and HeLa cells
-
Concentration:7-10 μM
-
Incubation Time:14 h
-
Result:Drastically reduced T120 phosphorylation.
-
Cell Line:RPE1 and HeLa cells
-
Concentration:0-30 μM
-
Incubation Time:1 h
-
Result:Reduced histone H2A-T120 phosphorylation.
Chemical Information
-
No. CAS 1445830-39-6
-
Appearance Solid
-
Peso molecular 482.48
-
Fòrmula C24H24F2N6O3
-
Color Off-white to light yellow
-
SMILES
COC1=CN=C(C2=NN(CC3=C(F)C=C(OCC)C=C3F)C(OC)=C2C)N=C1NC4=CC=NC=C4
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
-
Journal Impact Factor
-
Most Recent
Solvente y solubilidad
DMSO : 166.67 mg/mL (345.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 4.17 mg/mL (8.64 mM); Clear solution
This protocol yields a clear solution of ≥ 4.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (41.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
-
Ficha de datos (275 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0726 mL | 10.3631 mL | 20.7262 mL | 51.8156 mL |
| 5 mM | 0.4145 mL | 2.0726 mL | 4.1452 mL | 10.3631 mL | |
| 10 mM | 0.2073 mL | 1.0363 mL | 2.0726 mL | 5.1816 mL | |
| 15 mM | 0.1382 mL | 0.6909 mL | 1.3817 mL | 3.4544 mL | |
| 20 mM | 0.1036 mL | 0.5182 mL | 1.0363 mL | 2.5908 mL | |
| 25 mM | 0.0829 mL | 0.4145 mL | 0.8290 mL | 2.0726 mL | |
| 30 mM | 0.0691 mL | 0.3454 mL | 0.6909 mL | 1.7272 mL | |
| 40 mM | 0.0518 mL | 0.2591 mL | 0.5182 mL | 1.2954 mL | |
| 50 mM | 0.0415 mL | 0.2073 mL | 0.4145 mL | 1.0363 mL | |
| 60 mM | 0.0345 mL | 0.1727 mL | 0.3454 mL | 0.8636 mL | |
| 80 mM | 0.0259 mL | 0.1295 mL | 0.2591 mL | 0.6477 mL | |
| 100 mM | 0.0207 mL | 0.1036 mL | 0.2073 mL | 0.5182 mL |
BAY-524 Related Classifications
HY-104001 Related Classifications
Powered by Bioz