Elf18
Based on 1 Customer Validation
Elf18 is a peptide fragment of bacterial translation elongation factor Tu (EF-Tu). Elf18 can be recognized by plant pattern recognition receptors, thereby inducing an immune response. Elf18 can enhance plants' resistance to pathogens and can be used in research related to plant immune responses.
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- Pureza: 99.49%
- No. CAS: 849352-44-9
- Fòrmula: C91H149N27O28
- Peso molecular:2069.32
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Almacenamiento:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Descripciòn
In Vitro
Elf18 (300 nM; 30-90 min) can stimulate the production of H2O2 in Arabidopsis cells, but has no such effect in rice cells[1].
Elf18 (5 μM; 0-12 h) induces the expression of ELENA1 in 10-day-old wild-type Arabidopsis seedlings[2].
Elf18 (5 μM; 24 h) affects the expression of PR1 (pathogenesis related-gene 1) and PR2 (pathogenesis related-gene 2) in Arabidopsis plants with ELENA1 knockdown and overexpression[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:ELENA1 KD and OX arabidopsis
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Concentration:5 μM
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Incubation Time:24 h
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Result:Inhibited the expression levels of PR1 and PR2 in ELENA1 KD plants.
Increased the expression levels of PR1 and PR2 in ELENA1 OX plants.
Chemical Information
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No. CAS 849352-44-9
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Appearance Solid
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Peso molecular 2069.32
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Fòrmula C91H149N27O28
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Color White to off-white
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Sequence
Ac-Ser-Lys-Glu-Lys-Phe-Glu-Arg-Thr-Lys-Pro-His-Val-Asn-Val-Gly-Thr-Ile-Gly
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Sequence Shortening
Ac-SKEKFERTKPHVNVGTIG
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvente y solubilidad
In Vitro:
H2O : ≥ 100 mg/mL (48.33 mM)
DMSO : 25 mg/mL (12.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (281 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Furukawa T, et al., Two distinct EF-Tu epitopes induce immune responses in rice and Arabidopsis. Mol Plant Microbe Interact. 2014 Feb;27(2):113-24. [Content Brief]
[2]. Seo JS, et al. ELF18-INDUCED LONG-NONCODING RNA Associates with Mediator to Enhance Expression of Innate Immune Response Genes in Arabidopsis. Plant Cell. 2017 May;29(5):1024-1038. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 0.4833 mL | 2.4163 mL | 4.8325 mL | 12.0813 mL |
| 5 mM | 0.0967 mL | 0.4833 mL | 0.9665 mL | 2.4163 mL | |
| 10 mM | 0.0483 mL | 0.2416 mL | 0.4833 mL | 1.2081 mL | |
| H2O | 15 mM | 0.0322 mL | 0.1611 mL | 0.3222 mL | 0.8054 mL |
| 20 mM | 0.0242 mL | 0.1208 mL | 0.2416 mL | 0.6041 mL | |
| 25 mM | 0.0193 mL | 0.0967 mL | 0.1933 mL | 0.4833 mL | |
| 30 mM | 0.0161 mL | 0.0805 mL | 0.1611 mL | 0.4027 mL | |
| 40 mM | 0.0121 mL | 0.0604 mL | 0.1208 mL | 0.3020 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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