FM1-43 (solution)

Guide (The following is the experimental plan we recommend. This plan serves only as a reference guide. The specific operations should be adjusted according to your actual needs.)
1. FM Working Fluid Preparation Prepare the FM 1-43 working solution at a concentration of 5-20 μM using the preheated HBSS solution.
Note: Please adjust the concentration of the FM 1-43 working solution according to the actual situation, and prepare it as needed.

2. Cell Staining (Suspension Cells) 2.1 Centrifuge the cells and add PBS for two washes, each for 5 minutes. The cell density is 1×10⁶ cells/mL. 2.2 Add 1 mL of FM working solution and incubate at room temperature for 5 to 30 minutes.
2.3 400 grams. Centrifuge for 3-4 minutes. Discard the supernatant.
2.4 Add PBS to wash the cells twice, for 5 minutes each time.
2.5 After resuspending the cells in 1 mL of serum-free medium or PBS, observe them using a fluorescence microscope or a flow cytometer.
3. Cytological staining (adherent cells) 3.1 Cultivate adherent cells on a sterile cover glass.
3.2 Remove the cover glass from the culture medium and aspirate away the excess medium.
3.3 Add 100 μL of the dye working solution and gently shake to ensure complete coverage of the cells. Incubate for 5 to 30 minutes.
3.4 Remove the dye working solution and wash with the culture medium 2-3 times, for 5 minutes each time. Then observe using a fluorescence microscope or flow cytometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.