HJ-4
Based on 1 Customer Validation
HJ-4 is a Piperine (HY-N0144) derivative. HJ-4 potently inhibits the proliferation of CRC cells by dose-dependently reducing colony formation and DNA synthesis. HJ-4 markedly suppresses the adhesion, migration, invasion and induces apoptosis of CRC cells. HJ-4 demonstrates anti-tumor efficacy in chicken embryo chorioallantoic membrane (CAM) model implanted with HCT116/SW480 tumor spheroids. HJ-4 can be used for the study of colorectal cancer (CRC).
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.02%
- No. CAS: 1313741-00-2
- Fòrmula: C23H24N2O4
- Peso molecular:392.45
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Descripciòn
In Vitro
HJ-4 (3.125-100 μM, 24 h) inhibits viability of CRC cells (HCT116: IC50 = 15.82 μM; SW480: IC50 = 20.46 μM)[1].
HJ-4 (8-32 μM, 48 h) reduces colony number and size of HCT116/SW480 cells[1].
HJ-4 (8-32 μM, 24 h) decreases EdU-positive HCT116/SW480 cells in EdU incorporation assay, dose-dependently inhibiting DNA synthesis[1].
HJ-4 (8-32 μM, 0-48 h) inhibits adhesion, migration, and invasion of HCT116/SW480 cells[1].
HJ-4 (8-32 μM, 24 h) upregulates p53, PUMA, BAX, cleaved caspase-3, cleaved PARP, and downregulates BCL-2, β-catenin, Cyclin D1 in HCT116/SW480 cells[1].
HJ-4 (8-32 μM, 24 h) increases p53/BAX expression in HCT116/SW480 cells, and promotes YO-PRO-1 (early apoptosis) and PI (late apoptosis) positive cells in HCT116 cells via YO-PRO-1/PI staining[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HCT116/SW480 cells
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Concentration:8, 16, 32 μM
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Incubation Time:24 h
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Result:Upregulated p53, PUMA, BAX, cleaved caspase-3, cleaved PARP.
Downregulated BCL-2, β-catenin, Cyclin D1.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:HCT116/SW480 colon cancer cell-derived spheroids were implanted into the vascular-rich regions of the chorioallantoic membrane (CAM) of 7-day-old fertilized chicken embryos[1]
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Dosage:8, 16, 32 μM
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Administration:local application to tumor site, once daily for 7 days
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Result:Reduced tumor weight.
Showed potent anti-angiogenic effects in the CAM model.
Decreased the number of tertiary blood vessels.
Did not cause obvious embryonic toxicity in the CAM model.
Chemical Information
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No. CAS 1313741-00-2
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Appearance Solid
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Peso molecular 392.45
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Fòrmula C23H24N2O4
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Color yellow
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SMILES
COC1=CC=CC=C1N2CCN(CC2)C(/C=C/C=C/C3=CC4=C(OCO4)C=C3)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 4 mg/mL (10.19 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5481 mL | 12.7405 mL | 25.4810 mL | 63.7024 mL |
| 5 mM | 0.5096 mL | 2.5481 mL | 5.0962 mL | 12.7405 mL | |
| 10 mM | 0.2548 mL | 1.2740 mL | 2.5481 mL | 6.3702 mL |
HJ-4 Related Classifications
HY-178921 Related Classifications
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