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Referencia número: HY-D0121

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INDO 1

INDO 1 is a cell-impermeable, dual-emission ratiometric fluorescent calcium indicator with a K_D·β value of 0.44 μM. INDO 1 binds to intracellular free calcium ions and undergoes wavelength-dependent fluorescence changes. In the unbound state (free form) without Ca²⁺, its fluorescence emission peak is at 485 nm upon excitation with ultraviolet light (350 nm). When INDO 1 pentasodium binds to Ca²⁺ (bound state), the emission peak shifts to 405 nm (Ex = 350 nm; dual emission at Em= 405/485 nm).

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No. CAS: 96314-96-4
Fòrmula: C₃₂H₃₁N₃O₁₂
Peso molecular:649.60

Almacenamiento:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (274 KB)

INDO 1 Chemical Structure

No. CAS: 96314-96-4

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Actividad biológica
- Descripciòn
- In Vitro
- Emission (Em)
- Excitation (Ex)
Chemical Information
Pureza y Documentación
Referencias
Calculators
Help & FAQs
Classifications
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Actividad biológica

Descripciòn

In Vitro

INDO 1 enables accurate measurement of absolute cytoplasmic Ca²⁺ levels in R1-R6 photoreceptors of white-eyed Oregon wild-type Drosophila melanogaster^[1].
INDO 1 binds extensively to proteins isolated from rabbit ventricular myocytes^[2].
INDO 1 is a calcium-sensitive fluorescent dye used in glass capillaries, with an apparent K_D·β value of 0.44 μM; as calcium concentration increases, its emission at 405 nm enhances while emission at 470 nm diminishes^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emission (Em)

475

Excitation (Ex)

350

Chemical Information

No. CAS 96314-96-4
Peso molecular 649.60
Fòrmula C₃₂H₃₁N₃O₁₂
SMILES

O=C(C1=CC2=C(C=C1)C=C(C3=CC=C(N(CC(O)=O)CC(O)=O)C(OCCOC4=CC(C)=CC=C4N(CC(O)=O)CC(O)=O)=C3)N2)O
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (274 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Hardie RC, et al. INDO-1 measurements of absolute resting and light-induced Ca2+ concentration in Drosophila photoreceptors. The Journal of neuroscience : the official journal of the Society for Neuroscience. 1996 May 01;16(9):2924-33.

[2]. Hove-Madsen L, et al. Indo-1 binding to protein in permeabilized ventricular myocytes alters its spectral and Ca binding properties. Biophysical journal. 1992 Jul;63(1):89-97.

[3]. Jacquemond V, et al. Indo-1 fluorescence signals elicited by membrane depolarization in enzymatically isolated mouse skeletal muscle fibers. Biophysical journal. 1997 Aug;73(2):920-8.

Calculators

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

Minimum order quantity

100 mg

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