KLS-13019
Based on 1 Customer Validation
KLS-13019 is an orally active GPR55 receptor antagonist and a structural analogue of cannabidiol (CBD). It can prevent and reverse chemotherapy-induced peripheral neuropathy (CIPN) in a dose-dependent manner in rats.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.03%
- No. CAS: 1801243-39-9
- Fòrmula: C22H29NO3
- Peso molecular:355.47
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Almacenamiento:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Hippocampal neurone | EC50 |
0.04 μM
Compound: 16; KLS-13019
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Neuroprotective activity in rat hippocampal neurons assessed as protection against ammonium acetate induced cell death after 5 hrs by propidium iodide staining based fluorimetric analysis
Neuroprotective activity in rat hippocampal neurons assessed as protection against ammonium acetate induced cell death after 5 hrs by propidium iodide staining based fluorimetric analysis
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[PMID: 27096053] |
| Hippocampal neurone | EC50 |
0.04 μM
Compound: 16; KLS-13019
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Neuroprotective activity in rat hippocampal neurons assessed as protection against ammonium acetate induced cell death by measuring neuronal viability after 5 hrs by carboxyfluorescein diacetate dye based fluorimetric analysis
Neuroprotective activity in rat hippocampal neurons assessed as protection against ammonium acetate induced cell death by measuring neuronal viability after 5 hrs by carboxyfluorescein diacetate dye based fluorimetric analysis
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[PMID: 27096053] |
| Hippocampal neurone | EC50 |
0.05 μM
Compound: 16; KLS-13019
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Neuroprotective activity in rat hippocampal neurons assessed as protection against ethanol induced cell death after 5 hrs by propidium iodide staining based fluorimetric analysis
Neuroprotective activity in rat hippocampal neurons assessed as protection against ethanol induced cell death after 5 hrs by propidium iodide staining based fluorimetric analysis
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[PMID: 27096053] |
| Hippocampal neurone | EC50 |
0.08 μM
Compound: 16; KLS-13019
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Neuroprotective activity in rat hippocampal neurons assessed as protection against ethanol induced cell death by measuring neuronal viability after 5 hrs by carboxyfluorescein diacetate dye based fluorimetric analysis
Neuroprotective activity in rat hippocampal neurons assessed as protection against ethanol induced cell death by measuring neuronal viability after 5 hrs by carboxyfluorescein diacetate dye based fluorimetric analysis
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[PMID: 27096053] |
Chemical Information
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No. CAS 1801243-39-9
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Appearance Solid
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Peso molecular 355.47
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Fòrmula C22H29NO3
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Color Off-white to gray
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SMILES
CC([C@@H]1CCC(C)=C[C@H]1C2=C(C=C(C=C2O)CC3CN(C3)C(C)=O)O)=C
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (281.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (14.07 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (14.07 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8132 mL | 14.0659 mL | 28.1318 mL | 70.3294 mL |
| 5 mM | 0.5626 mL | 2.8132 mL | 5.6264 mL | 14.0659 mL | |
| 10 mM | 0.2813 mL | 1.4066 mL | 2.8132 mL | 7.0329 mL | |
| 15 mM | 0.1875 mL | 0.9377 mL | 1.8755 mL | 4.6886 mL | |
| 20 mM | 0.1407 mL | 0.7033 mL | 1.4066 mL | 3.5165 mL | |
| 25 mM | 0.1125 mL | 0.5626 mL | 1.1253 mL | 2.8132 mL | |
| 30 mM | 0.0938 mL | 0.4689 mL | 0.9377 mL | 2.3443 mL | |
| 40 mM | 0.0703 mL | 0.3516 mL | 0.7033 mL | 1.7582 mL | |
| 50 mM | 0.0563 mL | 0.2813 mL | 0.5626 mL | 1.4066 mL | |
| 60 mM | 0.0469 mL | 0.2344 mL | 0.4689 mL | 1.1722 mL | |
| 80 mM | 0.0352 mL | 0.1758 mL | 0.3516 mL | 0.8791 mL | |
| 100 mM | 0.0281 mL | 0.1407 mL | 0.2813 mL | 0.7033 mL |
KLS-13019 Related Classifications
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