country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

Cat. No.: HY-163858

Copy Link Print Information

Haspin-IN-4

Haspin-IN-4 (Compound 60) is a selective inhibitor of Haspin with an IC₅₀ value of 0.01 nM. Haspin-IN-4 possesses anticancer activity and can be used in cancer research.

For research use only. We do not sell to patients.

Formula: C₂₅H₃₃N₅O
Molecular Weight:419.56

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (266 KB)

Haspin-IN-4 Chemical Structure

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
Chemical Information
Protocols (1)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Haspin-IN-4 (Compound 60) is a selective inhibitor of Haspin with an IC₅₀ value of 0.01 nM. Haspin-IN-4 possesses anticancer activity and can be used in cancer research^[1].

IC₅₀ & Target

IC50: 0.01 nM (Haspin)^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	EC₅₀	24.31 μM Compound: 60	Cytotoxicity against human A-375 cells incubated for 48 to 72 hrs by MTT assay Cytotoxicity against human A-375 cells incubated for 48 to 72 hrs by MTT assay	[PMID: 39038808]
HeLa	EC₅₀	7.78 μM Compound: 60	Cytotoxicity against human HeLa cells incubated for 48 to 72 hrs by MTT assay Cytotoxicity against human HeLa cells incubated for 48 to 72 hrs by MTT assay	[PMID: 39038808]
IEC-6	EC₅₀	>40 μM Compound: 60	Cytotoxicity against rat IEC-6 cells incubated for 48 to 72 hrs by MTT assay Cytotoxicity against rat IEC-6 cells incubated for 48 to 72 hrs by MTT assay	[PMID: 39038808]
MCF7	EC₅₀	15.81 μM Compound: 60	Cytotoxicity against human MCF7 cells incubated for 48 to 72 hrs by MTT assay Cytotoxicity against human MCF7 cells incubated for 48 to 72 hrs by MTT assay	[PMID: 39038808]
MIA PaCa-2	EC₅₀	16.43 μM Compound: 60	Cytotoxicity against human MIA PaCa-2 cells incubated for 48 to 72 hrs by MTT assay Cytotoxicity against human MIA PaCa-2 cells incubated for 48 to 72 hrs by MTT assay	[PMID: 39038808]
Raji	EC₅₀	18.82 μM Compound: 60	Cytotoxicity against human Raji cells incubated for 48 to 72 hrs by MTT assay Cytotoxicity against human Raji cells incubated for 48 to 72 hrs by MTT assay	[PMID: 39038808]

Chemical Information

Molecular Weight 419.56
Formula C₂₅H₃₃N₅O
SMILES

CN1C=C(C2=CC(C(NC3=CC=C(C(C)=C3)C)=O)=CC=C21)CNCCN4CCNCC4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Cell Biology

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (266 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Vestuto V, et al. A Comprehensive In Vitro Characterization of a New Class of Indole-Based Compounds Developed as Selective Haspin Inhibitors. J Med Chem. 2024 Jul 22. [Content Brief]

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

Get Quote In-stock

Other size

Get Quote