HDAC8-IN-14

HDAC8-IN-14 (Compound 5e) (0.1-100 μM, 48 h) exhibits a low IC₅₀ value of 90 nM in MOLT-4 cells, along with IC₅₀ values of 1.98 μM in EL-4, 1.17 μM in YAC-1, and 18.94 μM in L1210 cells, while demonstrating a significantly higher IC₅₀ of 39.20 μM in the non-cancerous HEK-293 cell line, indicating its selectivity and safety toward non-cancerous cells at effective concentrations^[1].
HDAC8-IN-14 (100 nM, 30 min) shows significant ROS induction as measured by the fluorescent dye H2DCFDA (HY-D0940) in MOLT-4 cells^[1].
HDAC8-IN-14 (48 h) induces mitochondrial depolarization, evidenced by a shift in JC-1 fluorescence, triggers both apoptosis (~ 10 %) and necrosis (~ 30 %), and causes cell cycle arrest characterized by a 20 % increase in the sub-G0 apoptotic population alongside a reduction in G0/G1 and G2/M phases and a slight S-phase accumulation^[1].
HDAC8-IN-14 (50-100 nM, 48 h) induces a significant upregulation of cytochrome c, cleaved caspase-3, and the pro-apoptotic protein Bak while leaving the anti-apoptotic Bcl-2 levels unaltered, indicating that it activates the intrinsic apoptotic pathway through a Bcl-2-independent mechanism in MOLT-4 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.