HeFi-1
HeFi-1 is a mouse anti-CD30 IgG1 monoclonal antibody. HeFi-1 recognizes the ligand-binding site on CD30. HeFi-1 can inhibit the growth of tumor cells with high expression of CD30. HeFi-1 can induce eosinophil apoptosis. HeFi-1 can be used for researches on cancer or inflammation conditions such as anaplastic large-cell lymphoma (ALCL) and asthma.
For research use only. We do not sell to patients.
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Human
|
CD30 |
HeFi-1 (0.1 ng/μL, 1 h) can bind specifically to Karpas299 cells with a binding rate of over 90 %[1].
HeFi-1 (20 μg/mL, 48 h) can significantly inhibit the proliferation of Karpas299 cells and induces G1 phase blockade[1].
HeFi-1 (20 μg/mL, 12-24 h) can significantly induce apoptosis in eosinophil[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Karpas299 cells
-
Concentration:20 μg/mL
-
Incubation Time:48 h
-
Result:Significantly inhibited cell proliferation with an inhibition rate of 40%, superior to Daclizumab (HY-108738).
-
Cell Line:Karpas299 cells
-
Concentration:20 μg/mL
-
Incubation Time:48 h
-
Result:Induced G1 phase arrest and a decrease in S phase cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:1×107 Karpas299 cells injected SCID/NOD wild-type and FcRγ-/- mice[1]
-
Dosage:100 μg
-
Administration:Intravenous injection (i.v.), once weekly for 4 weeks
-
Result:Extended the median survival time to 50.5 days in SCID/NOD wild-type mice and 42.9 days in SCID/NOD FcRγ-/- mice, significantly higher than the control group.
Significantly reduced the level of sIL-2Rα.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
-
Product Image
ELISA, FACS, Functional assay
Chemical Information
-
SMILES
N/A
-
Formulation
Please refer to the lot-specific COA for specific buffer information.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Zhang M, et al. Effective therapy for a murine model of human anaplastic large-cell lymphoma with the anti-CD30 monoclonal antibody, HeFi-1, does not require activating Fc receptors. Blood. 2006 Jul 15;108(2):705-10. [Content Brief]
[2]. Berro AI, et al. Increased expression and activation of CD30 induce apoptosis in human blood eosinophils. J Immunol. 2004 Aug 1;173(3):2174-83. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
HeFi-1 Related Classifications
Cat. No.: HY-P991553
Powered by Bioz
-
Product Image