HSL-IN-1
Based on 3 publication(s) in Google Scholar
HSL-IN-1 (compound 24b) is a potent and orally active hormone sensitive lipase (HSL) inhibitor (IC50=2 nM) with a significantly reduced reactive metabolite liability.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.65%
- CAS 番号: 2095156-13-9
- 分子式: C19H13BClF3N2O4
- 分子量:436.58
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 HSL-IN-1
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生物活性
製品説明
IC50 & Target
IC50: 2 nM (Hormone sensitive lipase)[1]
体外実験
HSL-IN-1 (1 μM, 30 min) exhibits high metabolic stability in rat liver microsomes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Wistar rats (7-8 weeks old, 200-250 g)[1]
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Dosage:3 mg/kg
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Administration:Oral gavage (p.o.), single dose
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Result:Significantly reduced plasma glycerol levels. Cmax was 3.35 μg/mL, and AUC was 19.65 μg·h/mL.
化学情報
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CAS 番号 2095156-13-9
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性状 Solid
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分子量 436.58
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分子式 C19H13BClF3N2O4
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Color White to off-white
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SMILES
O=C(NC1=C(B(O)O)C=C(Cl)C=C1)C(C=C2)=CN=C2OC3=CC=C(C(F)(F)F)C=C3
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
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Most Recent
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Liver Int
Targeting ferroptosis alleviates methionine-choline deficient (MCD)-diet induced NASH by suppressing liver lipotoxicity. [Abstract]2020 Jun;40(6):1378-1394. PMID: 32145145 -
Commun Biol
Omega-6 highly unsaturated fatty acids in Leydig cells facilitate male sex hormone production. [Abstract]2022 Sep 21;5(1):1001. PMID: 36131086 -
Br J Nutr
The dysfunction of hormone-sensitive lipase induces lipid deposition and reprogramming of nutrient metabolism. [Abstract]2023 Aug 28;130(4):588-603. PMID: 36408747
溶剤 & 溶解度
体外:
DMSO : 12.5 mg/mL (28.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.86 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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データシート (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2905 mL | 11.4527 mL | 22.9053 mL | 57.2633 mL |
| 5 mM | 0.4581 mL | 2.2905 mL | 4.5811 mL | 11.4527 mL | |
| 10 mM | 0.2291 mL | 1.1453 mL | 2.2905 mL | 5.7263 mL | |
| 15 mM | 0.1527 mL | 0.7635 mL | 1.5270 mL | 3.8176 mL | |
| 20 mM | 0.1145 mL | 0.5726 mL | 1.1453 mL | 2.8632 mL | |
| 25 mM | 0.0916 mL | 0.4581 mL | 0.9162 mL | 2.2905 mL |
HSL-IN-1 関連分類
HY-101509 関連分類
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