HSP110-IN-1
HSP110-IN-1 is a HSP110 inhibitor. HSP110-IN-1 binds to HSP110, inhibits the activity of STAT3, and downregulates the expression of downstream genes VEGF, MMP7 and MMP9. HSP110-IN-1 abrogates IL-6-induced epithelial-mesenchymal transition and inhibits the proliferation of colorectal cancer cells. HSP110-IN-1 remodels the tumor microenvironment by inducing a pro-inflammatory phenotype, regulates macrophages, induces PD-L1 expression, and enhances anti-PD-L1 antibody-mediated tumor regression. HSP110-IN-1 can be used in studies related to colorectal cancer.
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- CAS 番号: 2991567-27-0
- 分子式: C29H32N4O5
- 分子量:516.59
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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生物活性
製品説明
IC50 & Target
[1]|
HSP110 |
STAT3 |
MMP-9 |
MMP-7 |
体外実験
HSP110-IN-1 (75 μM; 72 h) potently inhibits the viability of HCT116 and SW480 human colorectal cancer cells, with inhibition rates of 72.9% and 81.3%, respectively[1].
HSP110-IN-1 (0-500 μM; 48 h) inhibits the viability of HCT116 and SW480 human colorectal cancer cells in a dose-dependent manner, with IC50 values of 11.59 μM and 6.05 μM, respectively, and is more potent than the reference compound 1 at equivalent concentrations[1].
HSP110-IN-1 (0-10 μM; 24 h) dose-dependently inhibits STAT3 phosphorylation in HCT116 and SW480 human colorectal cancer cells, without affecting total STAT3 or HSP110 levels, and is more potent than the reference compound 1 at equivalent concentrations[1].
HSP110-IN-1 (5-10 μM; 24 h) downregulates the expression of STAT3 target genes MMP7, MMP9, and VEGF in HCT116 and SW480 human colorectal cancer cells, with greater potency than the reference compound 1 at equivalent concentrations[1].
HSP110-IN-1 (5-10 μM; 48 h) inhibits IL-6-induced migration of HCT116 and SW480 human colorectal cancer cells, with greater potency than the reference compound 1 at 10 μM[1].
HSP110-IN-1 (5-10 μM; 24 h) inhibits IL-6-induced invasion of HCT116 and SW480 human colorectal cancer cells, with greater potency than the reference compound 1 at 10 μM[1].
HSP110-IN-1 (5-10 μM; 24 h) attenuates IL-6-induced epithelial-mesenchymal transition in HCT116 and SW480 human colorectal cancer cells, with greater potency than the reference compound 1 at equivalent concentrations[1].
HSP110-IN-1 (40 μM; 96 h) inhibits the growth of human HCT 116 and HT-29 colorectal cancer 3D spheroids co-cultured with PBMCs[2].
HSP110-IN-1 (40 μM; 48 h) polarizes human M2-like macrophages toward a pro-inflammatory M1-like phenotype and enhances pro-inflammatory marker expression in human M1-like macrophages, while upregulating PD-L1 expression in both macrophage subsets[2].
HSP110-IN-1 (40 μM; 48 h) treatment of M2-like macrophages indirectly promotes proliferation and IFN-γ production in co-cultured autologous human CD8+ T lymphocytes, with this effect amplified by anti-PD-1 co-treatment, while having no direct effect on CD8+ T cell proliferation[2].
HSP110-IN-1 (40 μM; 48 h) treatment of human M1-like or M2-like macrophages enables these macrophages to inhibit the growth of co-cultured HT-29 colorectal cancer 3D spheroids, with enhanced inhibition in the presence of autologous activated T lymphocytes[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human colorectal cancer HCT116 and SW480 cells
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Concentration:75 μM
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Incubation Time:72 h
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Result:Produced the highest inhibition rate against HCT116 and SW480 cells among tested compounds, with an inhibition rate of 72.9% in HCT116 cells and 81.3% in SW480 cells.
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Cell Line:human colorectal cancer HCT116 and SW480 cells
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Concentration:0, 1.25, 2.5, 5 and 10 μM
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Incubation Time:24 h
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Result:Reduced phosphorylated STAT3 levels in a dose-dependent manner in both cell lines, without altering total STAT3 or HSP110 expression.
Inhibited STAT3 phosphorylation more significantly than the reference compound 1 at 5 and 10 μM.
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Cell Line:human colorectal cancer HCT116 and SW480 cells
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Concentration:5 and 10 μM
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Incubation Time:24 h
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Result:Substantially downregulated the expression of STAT3 downstream target genes MMP7, MMP9, and VEGF in both cell lines.
Exhibited more significant inhibitory effect than the reference compound 1 at 10 μM.
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Cell Line:IL-6-stimulated human colorectal cancer HCT116 and SW480 cells
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Concentration:5 and 10 μM (with 6.25 ng/mL IL-6 stimulation)
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Incubation Time:48 h
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Result:Suppressed IL-6-induced cell migration in a dose-dependent manner.
Exhibited more potent inhibitory effects on wound closure than the reference compound 1 at 10 μM.
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Cell Line:IL-6-stimulated human colorectal cancer HCT116 and SW480 cells
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Concentration:5 and 10 μM (with 6.25 ng/mL IL-6 stimulation)
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Incubation Time:24 h
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Result:Suppressed IL-6-induced cell invasion in a dose-dependent manner.
Exhibited more potent inhibitory effects on cell invasion than the reference compound 1 at 10 μM.
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Cell Line:IL-6-stimulated human colorectal cancer HCT116 and SW480 cells
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Concentration:5 and 10 μM (with 6.25 ng/mL IL-6 stimulation)
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Incubation Time:24 h
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Result:Increased the expression of epithelial marker E-cadherin and decreased the expression of mesenchymal markers N-cadherin and Snail in IL-6-stimulated cells.
Produced more significant reversal of EMT markers than the reference compound 1 at 5 and 10 μM.
Parmacokinetics
体内実験
HSP110-IN-1 (Compound C7) (5 mg/kg; i.p.; three times weekly; 21 days) induces a 75-80% reduction in CT26 colorectal tumor growth in BALB/c mice, reshapes the tumor microenvironment toward a pro-inflammatory state, and upregulates PD-L1 expression in multiple cell types without detectable toxicity at the tested dose[2].
HSP110-IN-1 (Compound C7) (5 mg/kg; i.p.; three times weekly; 21 days) potentiates the efficacy of anti-PD-L1 therapy in anti-PD-L1-resistant CT26 colorectal tumors in BALB/c mice, enhancing tumor regression, reshaping the tumor microenvironment, and promoting functional CD8+ tumor-infiltrating lymphocytes[2].
HSP110-IN-1 (Compound C7) (5 mg/kg; i.p.; three times weekly; 21 days) enhances the efficacy of anti-PD-L1 therapy in anti-PD-L1-sensitive MC38 colorectal tumors in C57BL/6 mice, driving superior tumor regression and reshaping the tumor microenvironment[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c nude mice (male, 4-6 weeks old, 18-23 g, HCT116 human colorectal cancer cell xenograft)[1]
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Dosage:5 mg/kg
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Administration:i.p.; every three days; 30 days
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Result:Reduced tumor volumes and weights significantly.
Reduced expression of STAT3 downstream genes VEGF and MMP9 significantly in tumor tissues.
Increased expression of tumor suppressor protein p53 significantly in tumor tissues.
Caused no significant changes in body weight.
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Animal Model:BALB/c (8-10-week-old female; subcutaneous injection of 5×105 CT26 wild-type cells)[2]
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Dosage:5 mg/kg
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Administration:i.p.; three times weekly; 21 days
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Result:Induced a 75-80% reduction in tumor growth compared to vehicle controls.
Increased tumor cell apoptosis and reduced phosphorylated STAT3 levels in tumor tissue compared to vehicle and first-generation inhibitor C33.
Reduced the expression of pro-tumoral M2-like tumor-associated macrophage marker CD206 without affecting M1-like marker CD80.
Increased PD-L1 expression in tumor-associated macrophages, tumor cells, myeloid-derived suppressor cells, and dendritic cells.
No toxicity detected via intestinal crypt apoptosis staining or body weight loss.\nSignificantly reduced tumor growth compared to vehicle.
When combined with anti-PD-L1, induced greater tumor regression than either monotherapy, including in the anti-PD-L1-resistant CT26 model.
Decreased CD206 expression in tumor-associated macrophages when used alone or in combination with anti-PD-L1.
Increased PD-L1 expression in tumor cells and M2-like macrophages, which was reduced by co-administration of anti-PD-L1.
Increased the percentage of CD8+ tumor-infiltrating lymphocytes co-expressing PD-1 and TIM-3 or PD-1 and LAG-3, which exhibited enhanced effector function (higher expression of granzyme B, IFN-γ, and IL-2) compared to monotherapies or vehicle.
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Animal Model:C57BL/6 (8-10-week-old female; subcutaneous injection of 5×105 MC38 cells)[2]
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Dosage:5 mg/kg
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Administration:i.p.; three times weekly; 21 days
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Result:Significantly reduced tumor growth compared to vehicle.
When combined with anti-PD-L1, induced greater tumor regression than either monotherapy in the anti-PD-L1-sensitive MC38 model.
Decreased CD206 expression in tumor-associated macrophages when used alone or in combination with anti-PD-L1.
Increased PD-L1 expression in tumor cells and M2-like macrophages, which was reduced by co-administration of anti-PD-L1.
化学情報
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CAS 番号 2991567-27-0
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分子量 516.59
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分子式 C29H32N4O5
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SMILES
COC1=CC=C(C=C1OC)N2CC3CN(CC3C2)C4=C(C=C(C=N4)C5=CC(OC)=C(C(OC)=C5)OC)C#N
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
HSP110-IN-1 関連分類
HY-184162 関連分類
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