Congo Red Staining Solution (1%)
Congo Red Staining Solution (1%) is an absorptive histochemical staining solution based on Congo Red (HY-D0236), which is specifically designed to detect amyloid deposits in tissue sections. When observed under a polarizing microscope, the stained amyloid complexes exhibit characteristic apple-green birefringence, making it a standard tool for the diagnosis of amyloidosis.
For research use only. We do not sell to patients.
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
In Vitro
Congo Red Staining Solution (1%) binds to amyloid aggregates, amyloid β-sheet structures, and self-aggregated major basic proteins through hydrophobic and electrostatic interactions, inducing absorption peak shift and abnormal birefringence under cross-polarized light[1][2].
Congo Red Staining Solution (1%) acts as a pH indicator, supports image-based extraction of eosinophil granules, and can be used for the identification of amyloid substances in vitro and in tissue sections[1][2].
Congo Red Staining Solution (1%) stains intact, cytolytic eosinophil granules and extracellular eosinophil granules, but does not stain diffuse/linear MBP deposits in cardiac tissue samples[2].
Congo Red Staining Solution (1%) binds non-amyloid substances, inhibits amyloid fibril formation and accumulation of protease-resistant PrP, disrupts preformed polyglutamine oligomers, and induces oligomerization of native proteins[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
-
Amyloid: Congo Red Amyloid Staining
Congo red amyloid staining is a histochemical method used to detect extracellular amyloid deposits in tissue sections based on the affinity of Congo red dye for β-pleated sheet-rich protein aggregates. When bound to amyloid, Congo red produces characteristic apple-green birefringence under polarized light microscopy, which is widely regarded as a diagnostic feature of amyloid deposition in histopathology. The diagnostic principle relies on the combination of dye binding (congophilia) and optical anisotropy under polarized illumination, which distinguishes amyloid from most non-amyloid eosinophilic extracellular deposits in routine histological evaluation. Amyloid identification by Congo red staining remains a cornerstone in diagnostic pathology despite the availability of adjunct methods such as immunohistochemistry and mass spectrometry, particularly because of its ability to localize deposits directly within tissue architecture. The specificity of Congo red-positive deposits is incre
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)