Pramiracetam
Based on 1 Customer Validation
Pramiracetam (CI-879 free base) is a PREP (prolyl endopeptidase) inhibitor. Pramiracetam improves cognitive impairment caused by traumatic brain injury. Pramiracetam can be used in the study of neurodegenerative diseases.
For research use only. We do not sell to patients.
- Purity: 98.21%
- CAS No.: 68497-62-1
- Formula: C14H27N3O2
- Molecular Weight:269.38
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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FDA Approved & Pharmacopeial Drug Library
HY-L066
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Neurodegenerative Disease-related Compound Library
HY-L086
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Heterocyclic Compound Library
HY-L138
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Off-patent Drug Library
HY-L141
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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HY-L035P
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FDA-Approved Drug Library Plus
HY-L022P
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HY-L022M
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Bioactive Compound Library
HY-L001
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HY-L205
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HY-L012
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HY-L022
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Drug Repurposing Compound Library
HY-L035
Biological Activity
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Long Evans hooded rats (250-300 g)[1]
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Dosage:7.5, 15 mg/kg
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Administration:i.p.
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Result:Improved performance in the reference memory component of the task, but did not significantly affect the working memory component.
Chemical Information
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CAS No. 68497-62-1
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Appearance Solid
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Molecular Weight 269.38
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Formula C14H27N3O2
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Color White to off-white
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SMILES
O=C(NCCN(C(C)C)C(C)C)CN1C(CCC1)=O
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Synonyms
CI-879 free base
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : ≥ 100 mg/mL (371.22 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 100 mg/mL (371.22 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.28 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.28 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 12.5 mg/mL (46.40 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Murray CL, et al. The effect of pramiracetam (CI-879) on the acquisition of a radial arm maze task. Psychopharmacology (Berl). 1986;89(3):378-81. [Content Brief]
[2]. Yoshimoto T, et al. Specific inhibitors for prolyl endopeptidase and their anti-amnesic effect. J Pharmacobiodyn. 1987 Dec;10(12):730-5. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 3.7122 mL | 18.5611 mL | 37.1223 mL | 92.8057 mL |
| 5 mM | 0.7424 mL | 3.7122 mL | 7.4245 mL | 18.5611 mL | |
| 10 mM | 0.3712 mL | 1.8561 mL | 3.7122 mL | 9.2806 mL | |
| 15 mM | 0.2475 mL | 1.2374 mL | 2.4748 mL | 6.1870 mL | |
| 20 mM | 0.1856 mL | 0.9281 mL | 1.8561 mL | 4.6403 mL | |
| 25 mM | 0.1485 mL | 0.7424 mL | 1.4849 mL | 3.7122 mL | |
| 30 mM | 0.1237 mL | 0.6187 mL | 1.2374 mL | 3.0935 mL | |
| 40 mM | 0.0928 mL | 0.4640 mL | 0.9281 mL | 2.3201 mL | |
| 50 mM | 0.0742 mL | 0.3712 mL | 0.7424 mL | 1.8561 mL | |
| 60 mM | 0.0619 mL | 0.3094 mL | 0.6187 mL | 1.5468 mL | |
| 80 mM | 0.0464 mL | 0.2320 mL | 0.4640 mL | 1.1601 mL | |
| 100 mM | 0.0371 mL | 0.1856 mL | 0.3712 mL | 0.9281 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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