Art. -Nr.: HY-W007376

Indole-3-carboxaldehyde

(Synonyms: 3-Formylindole)

4 Cited Publications

Kundenbewertung

Based on 4 publication(s) in Google Scholar

Indole-3-carboxaldehyde (3-Formylindole), a banlangen extract, is the product of the oxidative degradation of indole-3-acetic acid (IAA) by crude enzyme preparations from etiolated pea seedlings. Indole-3-carboxaldehyde is a biochemical used to prepare analogs of the indole phytoalexin cyclobrassinin. Indole-3-carboxaldehyde also enhances the epithelial barrier and anti-inflammatory activity in the intestinal tract.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 99.95%
CAS. Nr.: 487-89-8
Formel: C₉H₇NO
Molecular Weight:145.16

Speicherung:

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Indole-3-carboxaldehyde Chemical Structure

CAS. Nr.: 487-89-8

Größe

Preis

Verfügbarkeit

Menge

Amount: USD 0.00

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (4)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

Human Endogenous Metabolite

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
BV-2	IC₅₀	0.76 μM Compound: 11	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay	[PMID: 27919656]
HeLa	IC₅₀	>100 μM Compound: 7	Antiproliferative activity against human Hela cells assessed as reduction in cell viability measured after 48 hrs by MTT assay Antiproliferative activity against human Hela cells assessed as reduction in cell viability measured after 48 hrs by MTT assay	[PMID: 32531682]
HeLa S3	IC₅₀	>10 μM Compound: 8	Cytotoxicity against human HeLa S3 cells assessed as cell viability incubated for 48 hrs by microscopic analysis Cytotoxicity against human HeLa S3 cells assessed as cell viability incubated for 48 hrs by microscopic analysis	[PMID: 35704432]
L5178Y	IC₅₀	0.069 μM Compound: 18	Anticancer activity against mouse L5178Y assessed as cell growth inhibition by [3H]-thymidine assay Anticancer activity against mouse L5178Y assessed as cell growth inhibition by [3H]-thymidine assay	[PMID: 33940466]

In Vitro

Indole-3-carboxaldehyde (100 nM, 12 h) increases endothelial nitric oxide synthase (eNOS) transcription levels, reduces gene expression of VCAM, CCL2, and IL6, reduces ROS levels in HUVECs^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 487-89-8
Appearance Solid
Molecular Weight 145.16
Formel C₉H₇NO
Color Off-white to yellow
SMILES

O=CC1=CNC2=C1C=CC=C2
Synonyms

3-Formylindole
Structure Classification
- Alkaloids
- Indole Alkaloids
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Microorganisms
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Publications (4)

All Cell Proliferation/Viability AssayBio/Physico-chemical AssayCell Imaging/StainingRT-PCR

Sort by:

Journal Impact Factor

Phytomedicine

Saikosaponin A restores the IDO1-driven gut-testis kynurenine axis to alleviate oligozoospermia. [Abstract]

2026 Aug:158:158414. PMID: 42287814
Cell Prolif

Tryptophan Metabolic Enzyme IL4I1 Inhibits Ferroptosis by Decreasing Ubiquitination of Nrf2 via I3P in Glioblastoma. [Abstract]

2025 Jun;58(6):e13816. PMID: 40071723

Indole-3-carboxaldehyde purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2025 Jun;58(6):e13816. [Abstract]

Quantification of cell death in U87 and U251 cells treated for 24h with 10mM of Erastin or 1mM of RSL3 in the presence of 200mM of TRP, I3P, KYNA, Indole-3-carboxaldehyde (I3A), ILA, and IAA.

Indole-3-carboxaldehyde purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2025 Jun;58(6):e13816. [Abstract]

Indole-3-carboxaldehyde (I3A) did not block ROS and lipid peroxidation accumulation induced by 24h of Erastin treatment in U87 cells. ROS and lipid peroxidation quantification were determined by flow cytometry using the H2DCFDA and C11-BODIPY probe, respectively. Fer-1 was added as a positive control.

Indole-3-carboxaldehyde purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2025 Jun;58(6):e13816. [Abstract]

Indole-3-carboxaldehyde (I3A) did not block ROS and lipid peroxidation accumulation induced by 24h of Erastin treatment in U251 cells. ROS and lipid peroxidation quantification were determined by flow cytometry using the H2DCFDA and C11-BODIPY probe, respectively. Fer-1 was added as a positive control.
Chem Biol Interact

Fusobacterium nucleatum-derived 3-indolepropionic acid promotes colorectal cancer progression via aryl hydrocarbon receptor activation in macrophages. [Abstract]

2025 Jun 1:414:111495. PMID: 40174685
J Cell Mol Med

Indole-3-Carboxaldehyde Inhibits Inflammatory Response and Lipid Accumulation in Macrophages Through the miR-1271-5p/HDAC9 Pathway. [Abstract]

2024 Dec;28(24):e70263. PMID: 39698913

Indole-3-carboxaldehyde purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2024 Dec;28(24):e70263. [Abstract]

THP-1 macrophage-derived foam cells were treated with the indicated concentrations ofIndole-3-carboxaldehyde (ICA; 0-100 μM) for 24h or incubated with 50μM ICA (0, 6, 12, 24, 48 h) for different time points. CCK-8 was applied to measure cell viability.

Indole-3-carboxaldehyde purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2024 Dec;28(24):e70263. [Abstract]

Indole-3-carboxaldehyde (ICA; 50μM; 24 h) demonstrated that the intracellular lipid droplets in ICA group were smaller and fewer than those in control group in THP-1 macrophage-derived foam cells.

Indole-3-carboxaldehyde purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2024 Dec;28(24):e70263. [Abstract]

Indole-3-carboxaldehyde (ICA; 50μM; 24 h) could markedly suppress intracellular lipid deposition in THP-1 macrophage-derived foam cells. Representative images of Bodipy 493/503 staining and quantitative analysis.

Indole-3-carboxaldehyde purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2024 Dec;28(24):e70263. [Abstract]

THP-1 macrophage-derived foam cells were treated with Indole-3-carboxaldehyde (ICA; 50μM) or DMSO for 24h. (A, B) The expression of CD36 and SR-A was determined by qRT-PCR and western blot.

Indole-3-carboxaldehyde purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2024 Dec;28(24):e70263. [Abstract]

Indole-3-carboxaldehyde (ICA; 50μM; 24 h) showed no significant difference in Dil-ox-LDL uptake between ICA group and control group.

Indole-3-carboxaldehyde purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2024 Dec;28(24):e70263. [Abstract]

Indole-3-carboxaldehyde (ICA; 50 μM; 24 h) enhanced the capability of NBD-cholesterol efflux to apoA-I and HDL. Representative photomicrographs of NBDcholesterol burden and quantitative analyses of cholesterol efflux to apoA-I and HDL.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 20 mg/mL (137.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

6.8889 mL

34.4447 mL

68.8895 mL

5 mM

1.3778 mL

6.8889 mL

13.7779 mL

10 mM

0.6889 mL

3.4445 mL

6.8889 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2 mg/mL (13.78 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2 mg/mL.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2 mg/mL (13.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Robert E. Stutz, et al. Enzymatic Formation of Indole-3-Carboxaldehyde from Indole-3-Acetic Acid. Plant Physiol. 1958 May; 33(3): 207–212.

[2]. Lu Y, et al. Gut microbe-derived metabolite indole-3-carboxaldehyde alleviates atherosclerosis. Signal Transduct Target Ther. 2023 Oct 4;8(1):378. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	6.8889 mL	34.4447 mL	68.8895 mL	172.2237 mL
	5 mM	1.3778 mL	6.8889 mL	13.7779 mL	34.4447 mL
	10 mM	0.6889 mL	3.4445 mL	6.8889 mL	17.2224 mL
	15 mM	0.4593 mL	2.2963 mL	4.5926 mL	11.4816 mL
	20 mM	0.3444 mL	1.7222 mL	3.4445 mL	8.6112 mL
	25 mM	0.2756 mL	1.3778 mL	2.7556 mL	6.8889 mL
	30 mM	0.2296 mL	1.1482 mL	2.2963 mL	5.7408 mL
	40 mM	0.1722 mL	0.8611 mL	1.7222 mL	4.3056 mL
	50 mM	0.1378 mL	0.6889 mL	1.3778 mL	3.4445 mL
	60 mM	0.1148 mL	0.5741 mL	1.1482 mL	2.8704 mL
	80 mM	0.0861 mL	0.4306 mL	0.8611 mL	2.1528 mL
	100 mM	0.0689 mL	0.3444 mL	0.6889 mL	1.7222 mL

Keywords

Indole-3-carboxaldehyde

4 Cited Publications

100 mg Angebot einholen

Subscribe to the

MedChemExpress

Newsletter

Produktname:
Art. -Nr.:
Menge:
MCE Japan Authorized Agent:

Produktname:
CAS. Nr.:
Species:
Reinheit:
Menge:

Indole-3-carboxaldehyde

3-Indoleacetic acid

Indolelactic acid

3-Indolepropionic acid

Indole-3-pyruvic acid

Kynurenic acid

L-Tryptophan

Indole