Insecticidal agent 33 

Insecticidal agent 33 is an AChE inhibitor with nematicidal activity. Insecticidal agent 33 impairs locomotor activity of Caenorhabditis elegans, and induces oxidative damage in Caenorhabditis elegans, including elevated reactive oxygen species (ROS) levels, lipofuscin accumulation, lipid deposition, and disrupted cellular homeostasis. Insecticidal agent 33 can be used for the research of plant-parasitic nematode infection^[1].

Insecticidal agent 33 (compound A18) (48 h) potently kills Caenorhabditis elegans with an LC₅₀ of 4.19 mg/L, achieving 100% nematicidal activity^[1].
Insecticidal agent 33 (48 h) exhibits 65.5% nematicidal activity against Bursaphelenchus xylophilus^[1].
Insecticidal agent 33 (48 h) kills Meloidogyne incognita with an LC₅₀ of 21.15 mg/L, achieving 79.6% nematicidal activity that is comparable to positive control Tioxazafen (HY-136240)^[1].
Insecticidal agent 33 (50 mg/L) potently inhibits acetylcholinesterase activity in Caenorhabditis elegans^[1].
Insecticidal agent 33 binds to the active pocket of acetylcholinesterase via hydrogen bonds with residues Phe-288, Arg-289, and Ser-286, supporting its potent inhibitory activity against the enzyme^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Insecticidal agent 33 (compound A18) (5-100 mg/L; single treatment) impairs locomotor activity, and induces oxidative damage in C. elegans^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

389.43

C₂₁H₁₅N₃O₃S

COC1=CC=C2C(N=C(CO2)C3=CC=C(C=C3)C4=NOC(C5=CC=CS5)=N4)=C1

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Wu K, et al. Design, Synthesis, and Mechanism of Action Study of Novel Tioxazafen-Derived Nematicides Bearing Benzoxazine Moiety. J Agric Food Chem. 2026;74(21):16115-16123.  [Content Brief]