country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

Cat. No.: HY-112347

Copy Link Print Information

Isogranulatimide

Isogranulatimide is a Chk1 and GSK-3β inhibitor that reverses Etoposide (HY-13629)-induced G₂/M phase arrest by inhibiting G₂ DNA damage checkpoint signaling and enhances DNA damage-induced cytotoxicity in mp53 cancer cells. Isogranulatimide can be used for research on breast cancer.

For research use only. We do not sell to patients.

CAS No.: 244148-46-7
Formula: C₁₅H₈N₄O₂
Molecular Weight:276.25

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (280 KB)

Isogranulatimide Chemical Structure

CAS No.: 244148-46-7

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]^[3]

Chk1

GSK-3β

All Checkpoint Kinase (Chk) Isoforms :

All GSK-3 Isoforms :

In Vitro

Anti-cancerCell Cycle RegulationOthers

Anti-cancer

Cell Cycle Regulation

Isogranulatimide (compound IGR) (20 µM; 8 h) inhibits the G₂ DNA damage checkpoint in irradiated MCF-7 mp53 cells, releasing 23% of cells from G₂ phase arrest^[2].

Cell Cycle Regulation

Isogranulatimide (2.5-10 μM) reverses etoposide-induced G₂ phase arrest in vector FDC-P1 cells at 2.5 μM, but FDC-P1 cells overexpressing Mcl-1 require 10 μM to achieve comparable reversal^[1].

Others

Isogranulatimide (1 μM; 20 min) eliminates Mcl-1-associated CHKtide phosphorylation in etoposide-treated FDC-P1 cells, where 1 μM is sufficient to inhibit endogenous mouse Mcl-1-associated activity^[1].
Isogranulatimide selectively inhibits GSK-3β kinase activity in kinase assays^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Chemical Information

CAS No. 244148-46-7
Molecular Weight 276.25
Formula C₁₅H₈N₄O₂
SMILES

O=C(C1=C2C3=NC=CN3C(N4)=C1C5=C4C=CC=C5)NC2=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Jamil S, et al. An essential role for MCL-1 in ATR-mediated CHK1 phosphorylation. Molecular biology of the cell. 2008 Aug;19(8):3212-20.

[2]. Rundle NT, et al. G2 DNA damage checkpoint inhibition and antimitotic activity of 13-hydroxy-15-oxozoapatlin. The Journal of biological chemistry. 2001 Dec 21;276(51):48231-6.

[3]. Rundle N T. Checkpoint inhibition and cell cycle effects of 13-hydroxy-15-oxozoapatlin, ent-kaur-16-en-15-oxo-18-9oic acid, and isogranulatimide[D]. University of British Columbia, 2003.

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

Get Quote In-stock

Other size

Get Quote