Cat. No.: HY-W000701

Isourolithin A

Name: Isourolithin A
Brand: MedChemExpress
SKU: HY-W000701
Price: 130.00 USD
Availability: InStock
Rating: 5 (1 reviews)

(Synonyms: Iso-uroA)

Customer Review

Based on 1 Customer Validation

Isourolithin A (Iso-uroA) is a urolithin metabolite. Isourolithin A induces S-phase and G2/M-phase cell cycle arrest and apoptosis. Isourolithin A can be used for research on colon cancer.

For research use only. We do not sell to patients.

Purity : 98.32%
CAS No.: 174023-48-4
Formula: C₁₃H₈O₄
Molecular Weight:228.20

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Isourolithin A Chemical Structure

CAS No.: 174023-48-4

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

HY-126307
Urolithin B

NF-κB Inhibitor

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Isourolithin A (Iso-uroA) is a urolithin metabolite. Isourolithin A induces S-phase and G2/M-phase cell cycle arrest and apoptosis. Isourolithin A can be used for research on colon cancer^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
Caco-2	IC50	99.8 μM	Growth inhibition against human colon cancer Caco-2 cells by MTT assay after 24 hrs incubation. Growth inhibition against human colon cancer Caco-2 cells by MTT assay after 24 hrs incubation.	26680596
Caco-2	IC50	69.7 μM	Growth inhibition against human colon cancer Caco-2 cells by MTT assay after 48 hrs incubation. Growth inhibition against human colon cancer Caco-2 cells by MTT assay after 48 hrs incubation.	26680596
CCD-18Co	IC50	140.1 μM	Growth inhibition against normal human colon CCD18-Co cells by MTT assay after 24 hrs incubation. Growth inhibition against normal human colon CCD18-Co cells by MTT assay after 24 hrs incubation.	26680596
CCD-18Co	IC50	125.5 μM	Growth inhibition against normal human colon CCD18-Co cells by MTT assay after 48 hrs incubation. Growth inhibition against normal human colon CCD18-Co cells by MTT assay after 48 hrs incubation.	26680596
SW480	IC50	104.4 μM	Growth inhibition against human colon cancer SW480 cells by MTT assay after 24 hrs incubation. Growth inhibition against human colon cancer SW480 cells by MTT assay after 24 hrs incubation.	26680596
SW480	IC50	55.5 μM	Growth inhibition against human colon cancer SW480 cells by MTT assay after 48 hrs incubation. Growth inhibition against human colon cancer SW480 cells by MTT assay after 48 hrs incubation.	26680596
HT-29	IC50	111.4 μM	Growth inhibition against human colon cancer HT-29 cells by MTT assay after 24 hrs incubation. Growth inhibition against human colon cancer HT-29 cells by MTT assay after 24 hrs incubation.	26680596
HT-29	IC50	65.5 μM	Growth inhibition against human colon cancer HT-29 cells by MTT assay after 48 hrs incubation. Growth inhibition against human colon cancer HT-29 cells by MTT assay after 48 hrs incubation.	26680596

In Vitro

Anti-cancerApoptosisCell Cycle RegulationOthers

Anti-cancer

Isourolithin A (IsoUro-A) (50-100 µM; 24-48 h) inhibits the proliferation of Caco-2 colon cancer cells in a dose- and time-dependent manner, with an inhibition rate of >30% at 100 µM for 24 h and approximately 60% at 48 h^[2].
Isourolithin A inhibits Caco-2 growth, with an IC₅₀ value of 99.8 µM at 24 h and 69.7 µM at 48 h^[2].
Isourolithin A inhibits SW480 growth, with an IC₅₀ value of 104.4 µM at 24 h and 55.5 µM at 48 h^[2].
Isourolithin A inhibits HT-29 growth, with an IC₅₀ value of 111.4 µM at 24 h and 65.5 µM at 48 h^[2].

Anti-cancer

Apoptosis

Isourolithin A (50-100 µM; 24-48 h) induces apoptosis in Caco-2 cells, with approximately 20% apoptotic cells at 100 µM exposure for 24 h and approximately 29% at 48 h^[2].

Anti-cancer

Cell Cycle Regulation

Isourolithin A (50-100 µM; 24-48 h) arrests Caco-2 cells in S phase and G2/M phase after exposure at 50 and 100 µM for 24 h and 48 h^[2].

Apoptosis

Isourolithin A (50-100 µM; 24-48 h) does not increase apoptosis in normal CCD18-Co colon cells after exposure at 50 or 100 µM for 24 or 48 h^[2].

Cell Cycle Regulation

Isourolithin A (50-100 µM; 24-48 h) induces a slight G2/M phase arrest in CCD18-Co cells at 100 µM exposure for 24 h and at 50 and 100 µM exposure for 48 h, but does not cause S phase arrest^[2].

Others

Isourolithin A is formed from EA as a substrate by human fecal isolates CEBAS 4A1-CEBAS 4A4 in anaerobic culture and is the final urolithin metabolite^[1].
Isourolithin A inhibits CCD18-Co growth, with an IC₅₀ value of 140.1 µM at 24 h and 125.5 µM at 48 h^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:

Caco-2 cells
Concentration:

50 and 100 µM
Incubation Time:

24 and 48 h
Result:

Cell viability remained above 90% at the tested doses.
Inhibited Caco-2 proliferation in a concentration- and time-dependent manner at both 50 and 100 µM.
At 100 µM, inhibition was >30% at 24 h and about 60% at 48 h.

Cell Cycle Analysis^[2]

Cell Line:

Caco-2 cells
Concentration:

50 and 100 µM
Incubation Time:

24 and 48 h
Result:

Caused dose- and time-dependent arrest at S and G2/M phases after exposure to 50 and 100 µM at 24 and 48 h, with a decrease in G0/G1-phase cells.

Cell Cycle Analysis^[2]

Cell Line:

CCD18-Co cells
Concentration:

50 and 100 µM
Incubation Time:

24 and 48 h
Result:

At 100 µM, induced a slight G2/M-phase arrest at 24 h.
At 48 h, both 50 and 100 µM produced G2/M-phase arrest.
No S-phase arrest was observed after any exposure.

Apoptosis Analysis^[2]

Cell Line:

Caco-2 cells
Concentration:

50 and 100 µM
Incubation Time:

24 and 48 h
Result:

Increased early and late apoptotic Caco-2 cells in a concentration- and time-dependent manner, with effects detected at 100 µM at 24 h and at 50 and 100 µM at 48 h.
At 100 µM, apoptotic cells were about 20% at 24 h and about 29% at 48 h.

Apoptosis Analysis^[2]

Cell Line:

CCD18-Co cells
Concentration:

50 and 100 µM
Incubation Time:

24 and 48 h
Result:

Did not cause changes in the number of apoptotic CCD18-Co cells at the doses and times tested.

Related Protocols :

Chemical Information

CAS No. 174023-48-4
Appearance Solid
Molecular Weight 228.20
Formula C₁₃H₈O₄
Color White to off-white
SMILES

O=C1OC=2C=C(O)C=CC2C3=CC(O)=CC=C13
Synonyms

Iso-uroA
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 6.67 mg/mL (29.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.3821 mL

21.9106 mL

43.8212 mL

5 mM

0.8764 mL

4.3821 mL

8.7642 mL

10 mM

0.4382 mL

2.1911 mL

4.3821 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Selma MV, et al. Isolation of Human Intestinal Bacteria Capable of Producing the Bioactive Metabolite Isourolithin A from Ellagic Acid. Front Microbiol. 2017 Aug 7;8:1521.

[2]. González-Sarrías A, et al. Antiproliferative activity of the ellagic acid-derived gut microbiota isourolithin A and comparison with its urolithin A isomer: the role of cell metabolism. Eur J Nutr. 2017 Mar;56(2):831-841. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.3821 mL	21.9106 mL	43.8212 mL	109.5530 mL
	5 mM	0.8764 mL	4.3821 mL	8.7642 mL	21.9106 mL
	10 mM	0.4382 mL	2.1911 mL	4.3821 mL	10.9553 mL
	15 mM	0.2921 mL	1.4607 mL	2.9214 mL	7.3035 mL
	20 mM	0.2191 mL	1.0955 mL	2.1911 mL	5.4777 mL
	25 mM	0.1753 mL	0.8764 mL	1.7528 mL	4.3821 mL

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Isourolithin A