2-Iodoacetamide
Based on 9 publication(s) in Google Scholar
2-Iodoacetamide (Iodoacetamide), an alkylating agent, is a commonly used agent for alkylation of cysteine during sample preparation for proteomics.
For research use only. We do not sell to patients.
- Purity: 99.62%
- CAS No.: 144-48-9
- Formula: C2H4INO
- Molecular Weight:184.96
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) 2-Iodoacetamide
More- Nat Cell Biol. 2026 Apr;28(4):812-827. [Abstract]
- Nat Commun. 2026 Mar 13;17(1):3879. [Abstract]
- Cell Commun Signal. 2025 Feb 5;23(1):65. [Abstract]
- Commun Chem. 2025 Dec 9. [Abstract]
- J Enzyme Inhib Med Chem. 2025 Dec;40(1):2501743. [Abstract]
- Arab J Chem. 2024 Feb, 17(2), 105550.
- Sci Rep. 2026 Mar 20;16(1):14300. [Abstract]
- Biochim Biophys Acta Mol Cell Biol Lipids. 2024 Aug;1869(6):159513. [Abstract]
- STAR Protoc. 2025 Nov 3;6(4):104166. [Abstract]
Biological Activity
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Sprague-Dawley rats[2]
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Dosage:2.0-10 mg
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Administration:Drinking water, daily, for periods of 20 to 93 days
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Result:Produced gastritis which was further complicated by chronic ulceration in many of the test rats.
Chemical Information
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CAS No. 144-48-9
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Appearance Solid
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Molecular Weight 184.96
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Formula C2H4INO
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Color White to yellow
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SMILES
O=C(N)CI
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Synonyms
Iodoacetamide
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (9)
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Journal Impact Factor
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Most Recent
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Nat Cell Biol
TOLLIP targets GSDME-NT-carrying endocytic vesicles for autophagy to regulate pyroptosis and chemotherapy efficacy. [Abstract]2026 Apr;28(4):812-827. PMID: 41803502 -
Nat Commun
2026 Mar 13;17(1):3879. PMID: 41820364 -
Cell Commun Signal
Chemo-proteomics reveals dihydrocaffeic acid exhibits anti-inflammation effects via Transaldolase 1 mediated PERK-NF-κB pathway. [Abstract]2025 Feb 5;23(1):65. PMID: 39910568 -
Commun Chem
Stepped collisional energy improves protein acylation identification by eliminating positional bias of cyclic immonium ions. [Abstract]2025 Dec 9. PMID: 41366033 -
J Enzyme Inhib Med Chem
Natural product sennoside B disrupts liquid-liquid phase separation of SARS-CoV-2 nucleocapsid protein by inhibiting its RNA-binding activity. [Abstract]2025 Dec;40(1):2501743. PMID: 40371698 -
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Sci Rep
2026 Mar 20;16(1):14300. PMID: 41862552 -
Biochim Biophys Acta Mol Cell Biol Lipids
Proteomic and lipidomic landscape of the infrapatellar fat pad and its clinical significance in knee osteoarthritis. [Abstract]2024 Aug;1869(6):159513. PMID: 38788831 -
STAR Protoc
Protocol to quantify protein phosphorylation in mouse extended pluripotent stem cell-derived blastoids. [Abstract]2025 Nov 3;6(4):104166. PMID: 41191480
Solvent & Solubility
DMSO : 100 mg/mL (540.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (540.66 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.52 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.52 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (540.66 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (644 KB)
- English - EN (644 KB)
- Français - FR (644 KB)
- Deutsch - DE (644 KB)
- Norwegian - NO (644 KB)
- Español - ES (644 KB)
- Swedish - SV (644 KB)
- Italian - IT (644 KB)
- Korean - KR (644 KB)
- Portuguese - PT (644 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 5.4066 mL | 27.0329 mL | 54.0657 mL | 135.1644 mL |
| 5 mM | 1.0813 mL | 5.4066 mL | 10.8131 mL | 27.0329 mL | |
| 10 mM | 0.5407 mL | 2.7033 mL | 5.4066 mL | 13.5164 mL | |
| 15 mM | 0.3604 mL | 1.8022 mL | 3.6044 mL | 9.0110 mL | |
| 20 mM | 0.2703 mL | 1.3516 mL | 2.7033 mL | 6.7582 mL | |
| 25 mM | 0.2163 mL | 1.0813 mL | 2.1626 mL | 5.4066 mL | |
| 30 mM | 0.1802 mL | 0.9011 mL | 1.8022 mL | 4.5055 mL | |
| 40 mM | 0.1352 mL | 0.6758 mL | 1.3516 mL | 3.3791 mL | |
| 50 mM | 0.1081 mL | 0.5407 mL | 1.0813 mL | 2.7033 mL | |
| 60 mM | 0.0901 mL | 0.4505 mL | 0.9011 mL | 2.2527 mL | |
| 80 mM | 0.0676 mL | 0.3379 mL | 0.6758 mL | 1.6896 mL | |
| 100 mM | 0.0541 mL | 0.2703 mL | 0.5407 mL | 1.3516 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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