GC376 sodium
Based on 7 publication(s) in Google Scholar
GC376 sodium is a 3CLpro inhibitor; inhibits the replication of viruses TGEV, FIPV and PTV with IC50 values of 0.15, 0.2 and 0.15 μM, respectively.
For research use only. We do not sell to patients.
- Purity : 99.53%
- CAS No.: 1416992-39-6
- Formula: C21H30N3NaO8S
- Molecular Weight:507.53
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-143216
EnsitrelvirSARS-CoV-2 3CL Protease Inhibitor -
HY-138687
Nirmatrelvir3CLPRO Inhibitor -
HY-106161
RupintrivirHRV 3C Protease Inhibitor -
HY-100721R
GC376 sodium (Standard)3CLpro Inhibitor -
HY-N0017
BergeninBacterial Inhibitor -
HY-153377
Lipid 14Amino Lipid -
HY-N0017R
Bergenin (Standard)Bacterial Inhibitor
Publications Citing Use of MedChemExpress (MCE) GC376 sodium
More- Signal Transduct Target Ther. 2025 Dec 15;10(1):406. [Abstract]
- Acta Pharm Sin B. 2024 Sep;14(9):4028-4044. [Abstract]
- Nat Prod Bioprospect. 2025 Jun 13;15(1):39. [Abstract]
- Commun Biol. 2022 Sep 16;5(1):976. [Abstract]
- ACS Pharmacol Transl Sci. 2025 Oct 9;8(11):3944-3952. [Abstract]
- Vet Res. 2024 Sep 27;55(1):124. [Abstract]
- University of California. 2023 Jun.
Biological Activity
Description
IC50 & Target
IC50: 0.15 μM (TGEV), 0.2 μM (FIPV), 0.15 μM (PTV), 0.15 μM (229E), 1.1 μM (MHV), 5.3 μM (MNV-1), 0.6 μM (BCV)[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Vero C1008 | CC50 |
>100 μM
Compound: GC376
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Cytotoxicity against African green monkey GFP tagged Vero E6 cells assessed as reduction in cell viability by measuring fluoroscence in presence of CP1003 at 2 uM after 72 hrs
Cytotoxicity against African green monkey GFP tagged Vero E6 cells assessed as reduction in cell viability by measuring fluoroscence in presence of CP1003 at 2 uM after 72 hrs
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[PMID: 36332546] |
In Vitro
GC376 sodium covalently binds to Cys 139, Cys 147, and Cys 144 of NV 3CLpro, PV 3Cpro, and TGEV 3CLpro, respectively. GC376 sodium is significantly effective against caliciviruses (NV and MNV-1), coronaviruses (TGEV, FIPV, MHV, 229E, and BCV), and picornaviruses (HRVs 18, 51, and 68, EV71, and PTV), with nanomolar or low micromolar IC50s, except for FCV and HAV. Interestingly, FCV is less sensitive to GC376, with IC50 of 35 μM. GC376 sodium shows no or weak effectiveness against the replication of HAV in cells[1]. Proteases from NV, MD145 or MNV-1 are inhibited by GC376 sodium with a similar potency. The IC50 values of GC376 sodium against 3CLpro from NV, MD145, and MNV-1 are comparable among tested viruses[2]. GC376 sodium effectively inhibits the replication of NPI52-resistant viruses in cell culture as wild-type viruses, indicating that the mutation does not confer cross-resistance to GC376 sodium[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1416992-39-6
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Appearance Solid
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Molecular Weight 507.53
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Formula C21H30N3NaO8S
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Color Off-white to yellow
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SMILES
O=C(N[C@@H](CC(C)C)C(N[C@@](C(S(=O)(O[Na])=O)O)([H])CC1C(NCC1)=O)=O)OCC2=CC=CC=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (7)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Selective depletion of tumor-associated SAMHD1 enhances chemotherapeutic efficacy and antitumor immune responses. [Abstract]2025 Dec 15;10(1):406. PMID: 41392286 -
Acta Pharm Sin B
2024 Sep;14(9):4028-4044. PMID: 39309487 -
Nat Prod Bioprospect
Exploring anti-SARS-CoV-2 natural products: dual-viral target inhibition by delphinidin and the anti-coronaviral efficacy of deapio platycodin D. [Abstract]2025 Jun 13;15(1):39. PMID: 40512442 -
Commun Biol
Autoprocessing and oxyanion loop reorganization upon GC373 and nirmatrelvir binding of monomeric SARS-CoV-2 main protease catalytic domain. [Abstract]2022 Sep 16;5(1):976. PMID: 36114420 -
ACS Pharmacol Transl Sci
Development of a Cell-Based Recombinant Green Fluorescent Protein Assay System for Generalized Discovery of Viral Protease Inhibitors. [Abstract]2025 Oct 9;8(11):3944-3952. PMID: 41262580 -
Vet Res
Development and characterization of reverse genetics systems of feline infectious peritonitis virus for antiviral research. [Abstract]2024 Sep 27;55(1):124. PMID: 39334482 -
Solvent & Solubility
In Vitro:
H2O : ≥ 100 mg/mL (197.03 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Kim Y, et al. Broad-spectrum antivirals against 3C or 3C-like proteases of picornaviruses, noroviruses, and coronaviruses. J Virol. 2012 Nov;86(21):11754-62. [Content Brief]
[2]. Takahashi D, et al. Structural and inhibitor studies of norovirus 3C-like proteases. Virus Res. 2013 Dec 26;178(2):437-44. [Content Brief]
[3]. Yunjeong Kim, et al. Reversal of the Progression of Fatal Coronavirus Infection in Cats by a Broad-Spectrum Coronavirus Protease Inhibitor. PLoS Pathog. 2016 Mar 30;12(3):e1005531. [Content Brief]
[4]. Pedersen NC, et al. Efficacy of a 3C-like protease inhibitor in treating various forms of acquired feline infectious peritonitis. J Feline Med Surg. 2017 Sep 1:1098612X17729626. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.9703 mL | 9.8516 mL | 19.7033 mL | 49.2582 mL |
| 5 mM | 0.3941 mL | 1.9703 mL | 3.9407 mL | 9.8516 mL | |
| 10 mM | 0.1970 mL | 0.9852 mL | 1.9703 mL | 4.9258 mL | |
| 15 mM | 0.1314 mL | 0.6568 mL | 1.3136 mL | 3.2839 mL | |
| 20 mM | 0.0985 mL | 0.4926 mL | 0.9852 mL | 2.4629 mL | |
| 25 mM | 0.0788 mL | 0.3941 mL | 0.7881 mL | 1.9703 mL | |
| 30 mM | 0.0657 mL | 0.3284 mL | 0.6568 mL | 1.6419 mL | |
| 40 mM | 0.0493 mL | 0.2463 mL | 0.4926 mL | 1.2315 mL | |
| 50 mM | 0.0394 mL | 0.1970 mL | 0.3941 mL | 0.9852 mL | |
| 60 mM | 0.0328 mL | 0.1642 mL | 0.3284 mL | 0.8210 mL | |
| 80 mM | 0.0246 mL | 0.1231 mL | 0.2463 mL | 0.6157 mL | |
| 100 mM | 0.0197 mL | 0.0985 mL | 0.1970 mL | 0.4926 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
GC376 sodium Related Classifications
HY-100721 Related Classifications
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